15
4s3™ Semi-Automatic Sheet Heat Sealer
4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0
4.2 Plate Support Adapters
The 4s3™ is designed to seal microplates from 4 mm to 60 mm in height. All plates must first be placed in an
appropriate adapter to bring the sealing surface into the correct height range for the sealer. In addition to this, 96
and 384 well PCR plates require an adapter to support the plate precisely enough to produce an even seal. With
a 96 and 384 well PCR plate it is necessary to support each well completely, whereas with standard microplates,
it is only necessary to support the underside of the well but not the skirt.
Our Random Access plates supply a novel 96 well plate design with individually removable tubes. These plates
can be sealed in one step using Random Access heat seals - 96 individual foil seal spots on a removable backing
- resulting in individually sealed tubes. Please refer to the table to select the correct adapter for the plate you wish
to seal.
Plate Type
Adapter to use
Fully skirted PCR plates
(e.g. 384 well and low profile
96 well PCR Plates, fully skirted
FrameStar
®
PCR plates)
Plate Support Adapter, Standard
(4ti-0615, included)
4ti-0615
96 well PCR plates
Plate Support Adapter, PCR 96
(4ti-0625, optional extra;
includes 4s3™ Sealing Frame 4ti-0612)
Note: When using the PCR 96 adapter, the standard plate
adapter (4ti-0615, included) should be removed from the drawer.
4ti-0625
Fully skirted 1-component 384
well PCR plates (FrameStar
®
384 well plates also compatible)
Plate Support Adapter, PCR 384
(4ti-0618, optional extra)
4ti-0618
Shallow well plates
(e.g. FrameStar
®
384 Well PCR
Plates)
Plate Support Adapter, Standard
(4ti-0615, included)
placed inside
Plate Support Adapter, PCR 96
(4ti-0625, optional extra)
Note: This set-up allows the use of the 4s3
™
Sealing Frame
(4ti-0612) to keep the seal perfectly aligned.
4ti-0615 + 4ti-0625
Roche 1536 well plates
Plate Support Adapter, Roche 1536
(4ti-0616, optional extra)
placed inside
Plate Support Adapter, PCR 96
(4ti-0625, optional extra)
4ti-0616 + 4ti-0625
Deep well plates, fully skirted
SBS standard plates (except 96
well PCR plates)
Plate Support Adapter, Storage 96 and 384
(4ti-0617, optional extra)
4ti-0617
Use of Random Access Heat
Seals with low profile plates
Plate Support Adapter, Random Access, Low Profile
(4ti-0613, optional extra)
4ti-0613
Use of Random Access Heat
Seals with standard profile
plates
Plate Support Adapter, Random Access, Standard Profile
(4ti-0614, optional extra)
4ti-0614
Use of Random Access Heat
Seals with standard profile
FrameStar
®
Break-2-Ways Plates
Plate Support Adapter, Random Access, Standard Profile
Break-2-Ways
(4ti-0614-1, optional extra)
4ti-0614-1