4titude 4s3, Operation Manual

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4s3™ Semi-Automatic Sheet Heat Sealer
4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0
4.4.1 Sealing Parameters
Note: These values are for guidance only. It is advisable to conduct trials depending on the plate
type being sealed. Add 1 second to the sealing time when the 4s3
™
Weighted Sealing Platen is used
(4ti-0602)
Code Description
Sealing temperature (°C) / Dwell time (sec)
96 well PCR plates* 384 well PCR plates* Vision Plates ™ **
4ti-0541 Clear Heat Seal 175-185 / 2-3 165-180 / 3 185-200 / 3
4ti-0575 Clear Weld Heat Seal Mark 2 175-180 / 2-3 170-175 / 2-3
4ti-0581 Clear Heat Seal 3730 165-175 / 3 165-175 / 2 175-185 / 2-3
4ti-05481 Clear Heat Seal Plus 175-185 / 2-3 165-180 / 3 185-200 / 3
4ti-0521 Peel Heat Seal 175-185 / 3 170-175 / 2-3
4ti-05231 Universal Peel Heat Seal 175 / 2 175 / 2 180 / 2
4ti-0587 DMSO Resistant Peel Heat Seal 175-185 / 3 170-175 / 2-3
4ti-0531 Pierce Heat Seal 160-175 / 2 160-175 / 2 185-200 / 3
4ti-05381 Pierce Heat Seal Strong 170-180 / 2 170-180 / 2 180-200 / 3
4ti-0536 Foil Heat Seal 165-180 / 2 160-175 / 2-3 185-200 / 3
4ti-0547 Polystyrene Foil Heat Seal 185-200 / 3
4ti-0591 Thermal Bond Heat Seal 170-180 / 2-3 160-170 / 2
4ti-0595 Gas Permeable Heat Seal Mark 2 165-175 / 3 165-175 / 3 175-185 / 3
4ti-0541/SLIT Gas Permeable Clear Heat Seal 175-185 / 2-3 165-180 / 3 185-200 / 3
4ti-0543 96 INDI™ Heat Seal 175 / 3
* These values are for two-component PCR plates. For one-piece polypropylene plates, increase dwell time by one second
** These values can be used for all polystyrene plates
4.4.2 Sealing Parameters Random Access Seals
Note: These values are for guidance only. It is advisable to conduct trials depending on the plate type
being sealed.
Code Description
Sealing temperature (°C) / Dwell time (sec)
Plate Support Adapter required for low / standard profile plate
Random Access
Plates
4ti-0613 / 4ti-0614
FrameStar ®
Break-A-Way Plates
4ti-0613* / 4ti-0614
FrameStar ®
Break-2-Ways Plates
4ti-0613** / 4ti-0614-1
4ti-0521/RA-TAB Random Access Peel Heat Seal,
With Tabs 180 / 3 185 / 4 185 / 4*
4ti-0521/RA-8 Random Access Peel Heat Seal
96/8 n/a 185 / 4 185 / 4*
4ti-05381/RA Random Access Pierce Heat Seal
Strong 185 / 5 185 / 5 185 / 5*
* Applies to FrameStar ® Break-A-Way Plates, Low Profile with a lot no. >504035. Plates with a lot no. <504035 will be
compatible with adapter 4ti-0613-1 (for details please contact us at 4TI-TechnicalSupport@brooks.com).
** For FrameStar
® Break-2-Ways Plates, Low Profile , increase dwell time by one second.