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Introduction

Congratulations on your purchase of the Desktop Microscope. Your microscope comes from a
line of instruments that all operate under proprietary digital inline holography technology plat-
form, developed by 4Deep. With its new and unique method of data acquisition and analysis,
this system is designed to optimize your research of the microscopic world.

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General Safety Information

This instrument is of safety class 1 and has been built and tested according to EN 61010
1/IEC 1010-1/VDE 0411-1 safety standards for electrical measurement, control and laboratory
equipment.

Attention!

This microscope contains a class IIIB Laser. Up to 100 mW at 405 nm is emitted from the laser
housing itself. If properly assembled, the light emitted from the pinhole should not pose any
threat of long term damage to an exposed eye. There are no user serviceable parts inside the
light source. If problems are encountered regarding the light source, please return it imme-
diately for service. Allow only a qualified technician to disassemble the point source.

Do not

attempt to remedy any problems with the light source on your own!

Attention!

Always make sure that the proper 12 VDC power supply is used with the microscope. Failure
to do so could result in critical damage to the microscope.

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What’s in the Box

The following is the list of parts shipped as part of the Desktop microscope.

1. Desktop microscope, pre-assembled. See the microscope description in the “Microscope

and its Components” section. Follow the cable connection instructions in the “Microscope
Setup” section.

2. Power supply (12 VDC).

3. Ethernet cable.

4. Sample holder.

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The Microscope and its Components

The Desktop microscope consists of 3 primary components:

1. Camera: Housed within the top portion of the microscope.

2. Sample Stage: An x-y-z translation stage

3. Point Source: A laser and pinhole, located in the bottom portion of the microscope.

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Summary of Contents for Desktop Microscope

Page 1: ...Desktop Microscope User Guide Version 1 1 0 4Deep inwater imaging 6589 Chebucto Road Halifax Nova Scotia B3L 1L9 Canada tel 902 444 4449 info 4 deep com www 4 deep com 1...

Page 2: ...oscope and its Components 3 4 1 Camera 4 4 2 Sample Stage 5 4 3 Point Source 5 5 Microscope Setup 6 5 1 Step 1 6 5 2 Step 2 6 5 3 Step 3 6 6 Principle of Operation 6 7 Software 7 8 Tips for Optimal Ho...

Page 3: ...viceable parts inside the light source If problems are encountered regarding the light source please return it imme diately for service Allow only a qualified technician to disassemble the point sourc...

Page 4: ...rolled by the camera vertical control knob shown in Fig 2 This alters the Source to Screen Distance SSD which changes the magnification and resolution of the microscope Increase SSD Magnification Incr...

Page 5: ...ject Distance SOD and the vertical position of the sample stage controls this Warning Be careful of the point source when moving the vertical sample stage position DO NOT touch the point source a The...

Page 6: ...h the switch shown in Fig 4 on the previous page 5 3 Step 3 Open the Octopus program and go to the camera tab The program connects to the camera automatically and holograms can be saved or reconstruct...

Page 7: ...scope camera at the rate of up to 15 frames per second Octopus provides advanced 3D visualization and particle detection capabilities Stingray is an automated particle recognition and morphology class...

Page 8: ...containing only the light from the PS without a sample object causing scatter A good background image will allow for background subtraction when reconstructing holograms producing higher contrast rec...

Page 9: ...erating system Windows XP or later Communication power Ethhernet 12 VDC Hologram size from 2048x2048 to 512x512 Frame rate from 16 to 50 fps List of Figures 1 Main view of the Desktop microscope 4 2 T...

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