b. ZEUS ELECTRO DISH
Electro Dish (ED) is a specially designed disposable sterile petri dish with
metal electrodes and four separate pools between which the cells are
placed (Figure 6-A). There are points of electrode cables connection com-
ing out of the device between the two bridges at the front of the petri dish
(Figure 6-B).
c. ZEUS DISH FASTENER
Dish Fastener (DF) is a metal block designed to fit in the channels on the
sides of the petri dish (ZEUS
Electro Dish) and made for
keeping the petri dish stable
during processing. It also acts
as a thermal block. Due to its
U-shaped structure, it enables
the petri dish to be viewed un-
der a microscope (Figure 7).
d. ZEUS ELECTROLIQUID
Electroliquid Solution (ES) is a sterile electrolyte solution prepared to
transmit electric current to the cell. It contains Mannitol solution, MgS04,
CaCl2, HSA and HEPES. Electroliquid vials should be stored at 2-8 ° C.
Electrolyte concentrations in this product are very sensitive and it is sta-
ble until the expiration date shown on the vial label.
e. ZEUS ELECTROLIQUID PREPARATION & PIEZOELECTRIC
APPLICATION
i. APPLICATION PROTOCOL
1. Prior to use, Electroliquid Solution (ES) should be heated up to 37° for
a minimum of 30 minutes.
2. The channels on Electro Dish (ED) should be filled with 30 µl heated
ES with a micropipette.
3. As shown on Figure 8, on the ED 4 40-µl ES washing droplets are
made.
4. 20-30 minutes after ICSI procedure, the oocytes are taken from the
culture medium, first they are transported to the first wash droplet
and washed by pipetting, and then they are washed by pipetting at
the 2nd, 3rd and 4th droplets. Ensure that oocytes are always on the
droplet floor while washing.
5. After washing (gradual adaptation) oocytes are placed in the pools
filled with ES solution (Figure 8). Cells should be placed in the midline
of the channel within equal distance from the electrode rods (Figure
8/3-A).
USER
MANUAL
6. After placing the cells in the channels, ED is placed on an inverted
microscope and fixed by means of “Dish Fastener” (DF). Then, using
clamps the connection is made to the appropriate electrode poles (Fig-
ure 6-B).
7. During the procedure, continue following the cells under an inverted or
stereo microscope. Apply electrical stimulation according to Device Us-
age Instructions within Application Instructions. At this stage, Dish Fas-
tener can be used to fix the petri dish (ED) under a microscope (Figure 7).
8. Immediately after electrostimulation, aspirate the oocytes from the
electrode channels and transfer them to the standard culture dish,
wash with culture medium and follow the traditional embryo culture
procedure.
ii. ELECTROLIQUID STORAGE CONDITIONS AND STABILITY
Electroliquid Solution (ES) vial at 2-8 ° C.
This product is stable until the expiration date shown on the label on the
vial.
iii. MATTERS TO TAKE INTRO CONSIDERATION
• Do not resterilize, do not reuse.
• Do not use blurred or yellowed solutions.
• If you notice anything unusual about the features on the label, do not
use the product.
• Do not use if the sterile ED package or ES vial has been opened or
damaged.
• Products should be stored in their original unopened packaging at
2-8° C after delivery.
• Do not use the product after expiration date.
• Aseptic techniques should be used during procedures.
• Use only sterilized equipment and materials.
• Follow all local environmental regulations when disposing.
• Read the instructions for use before use.
iv. OPERATING THE DEVICE (Electrical Stimulation)
Connect the device to an earthed wall socket using the provided adapt-
er (adapter input), and connect the electrode cable to the electrode in-
put. After the connections are made, you can operate the device from
the ON / OFF button. When the device is turned on, first the opening
screen and then the application screen will appear (Figure 2 and 3). As of
this step, follow the directions at the bottom of the screen, first select
the type of impulse (DC or AC / DC mode), then follow the directions and
perform the application with the LOAD and then APPLY buttons. At any
point, press the BACK button to return to the previous screen.
Figure 6 - A
Figure 6 - B
Figure 8
PULSE STIMULATOR (ZPS 19)
b i o s c i e n c e
ZEUS Bioscience
+90 312 427 4801
[email protected]
www.zeusbioscience.com
