Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
10/2014 V_01 000000-2071-464 313
5.4.9 Processing – Ion Concentration
Licensing Instrument
standard
 optional  LSM
ELYRA Lightsheet Z.1
Tool accepts single-channel or multi-channel, multi-dimensional data (typically time
series data) of samples labeled with fluorescent ion indicators.
Ion Concentration was
developed for analyzing LSM data. Successful analysis of image data obtained with
other systems is potentially possible as well (provided the input meets the
requirements for intensity quantification). See full description of tool for details.
This Processing function permits the calibration of ion
concentrations in physiological experiments.
To open the
Ion Concentration
tools click
Ion
concentration (Fig. 414).
The two buttons select the active image in the
Image Display
as
Input Image
and
Input Image 2
(for
background subtraction). The name of the automatically
generated Output Image is shown under the name of
the Input Images.
In the
Preview
panel at the bottom of the Ion
concentration tool window, thumbnails of the input
images as well as a preview of the output image are
displayed (Fig. 415).
After opening the
Method Parameters
panel full
window by clicking the little arrow, the full tool window
opens and the following functions are available
(Fig. 416).
Opens the display for the resulting calibration curve.
buttons
To load & save calibration curves from / to files.
tick box
To select Input Image 2 as background image.
Fig. 414 Processing – Ion
concentration
Fig. 415 Ion concentration panel