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Zeiss LSM 880 Скачать руководство пользователя страница 27

CHAPTER 1 - NOTES ON DEVICE SAFETY

LSM 880

Notes on Handling the Laser Components and Illumination Systems

ZEISS

11/2014 V_05

000000-2071-466

25

When using a high power broadband illumination (HBO, X-Cite, HXP) in combination with a

neutral density reflector module, or in combination with a reflectorized object slide and
reflector modules without excitation filter and emission filter in the incident light path, there are

operating conditions which lead to strong reflections of excitation light and strong optical

radiation into the eyepiece. According to regulations for radiation load the maximal values are
below the permissible threshold, and do not lead in all probability to any permanent irritation of

the eyes, also because the natural averting reaction limits the exposure to the bright light.
Nevertheless we recommend not looking through the eyepiece when using the described setup

and when rotating the reflector turret. Especially, we do not recommend the use of the
reflector turret controls located on the microscope stand, since this leads to an observation

position close to the eyepiece. Instead, use the TFT-Touch screen or the LSM software to switch

the reflector turret. In case the reflector turret has to be rotated by hand avoid looking into the
eyepiece while doing so.

For the NLO system equipped with a specific push and click filter for NDD imaging be aware
that the NDD reflector cube in the reflector turret leads to a strong back reflection of HBO light

into the specimen plane and the eyepiece. When observing the specimen through the ocular
lens the use of the NDD reflector cube should be avoided. The light flash is not harmful but

unpleasant. The reflex of closing the eyelid is sufficiently protective.

Notes on the fiber used with the laser module UV (355 nm)

This component is a wear part and needs special handling to protect it from damage, e.g. don’t use the

maximum power level of the laser together with max. AOM transmission for dye excitation. For ROI
photo manipulation, elevated power levels should only be used for short time. Such laser powers are not

useful from an applicative point of view too, since photo damage might occur to biological tissue.
A replacement fiber can be ordered at your local ZEISS sales or service representative using order #

000000-1870-253 (UV-Fiber, packed). The replacement fiber must be installed by a ZEISS service

representative. We recommend to replace the fiber when the power is decreasing by more than 25 %.

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Содержание LSM 880

Страница 1: ...LSM 880 and LSM 880 NLO Notes on device safety and installation requirements November 2014...

Страница 2: ...pecifications are subject to change the manual is not covered by an update service Unless expressly authorized forwarding and duplication of this document and the utilization and communication of its...

Страница 3: ...g the Laser Components and Illumination Systems 23 9 Notes on Care Maintenance and Service 26 9 1 Exchanging Fuses 26 Cleaning 27 9 2 27 9 3 Maintenance of the Liquid Cooling System 27 9 4 Count on Se...

Страница 4: ...a at the base port of Axio Observer Z1 SP stands with motorized beam path switching is not allowed for reasons of laser safety Any manipulation will result in the loss of warranty of laser safety Plea...

Страница 5: ...ol and laboratory use EMC requirements Low voltage directive 2006 95 EC EMC directive 2004 108 EC KN 61000 6 2 KN 11 EMC User notice for Korean only A A The company works according to a certified Envi...

Страница 6: ...ding to DIN EN 61326 1 05 2010 1 Noise emission according to CISPR 11 DIN EN 55011 04 2011 2 Noise immunity according to table 2 industrial sector The LSM contains sensitive electronics A few seconds...

Страница 7: ...2071 466 5 The Ti Sa laser s and OPO need to have a separate electrical connection according to the instructions of the manufacturer When operating the main switch of the LSM 880 NLO the Ti Sa lasers...

Страница 8: ...6 000000 2071 466 11 2014 V_05 Fig 2 Power connector for the LSM 880 system including NLO system and set ups with laser module UV and components Free reserve outlets may be used to supply power to add...

Страница 9: ...nterlock at the same position Two door interlocks can be connected The LSM 880 system is controlled by a remote control This remote control contains the main switch for the system and the key switch f...

Страница 10: ...NLO for Mai Tai Laser or Chameleon 180 150 75 200 Active anti vibration table NLO for two microscope configuration 250 150 75 400 Periscope for two NLO lasers or NLO laser with OPO 40 37 70 40 Active...

Страница 11: ...15 C to 35 C any conditions different from item 1 and 5 3 Storage less than 16 h T 20 C to 55 C 4 Storage less than 6 h T 20 C to 55 C 5 Temperature gradient 0 5 C h 6 Warm up time 1 h for high precis...

Страница 12: ...s supplied Continuity of PE connection must not be affected by the use of extension leads The system contains components with dangerous voltage The system must not be opened by anybody else than autho...

Страница 13: ...e lamp After installation or conversion of the LSM system authorized specialized staff must carefully check that it is in a proper condition and particularly that covers protecting against laser radia...

Страница 14: ...s Caution Faults and hazards that might arise during operation which might cause damage to the unit or injury to the user Caution Laser radiation hazards possible when operating the system Caution Opt...

Страница 15: ...1 NOTES ON DEVICE SAFETY LSM 880 Warning and Information Labels ZEISS 11 2014 V_05 000000 2071 466 13 Fig 5 Warning and information labels on the Axio Observer Z1 microscope with the LSM 880 scanning...

Страница 16: ...ON DEVICE SAFETY ZEISS Warning and Information Labels LSM 880 14 000000 2071 466 11 2014 V_05 Fig 6 Warning and information labels on the Axio Observer Z1 microscope with the LSM 880 scanning module...

Страница 17: ...S ON DEVICE SAFETY LSM 880 Warning and Information Labels ZEISS 11 2014 V_05 000000 2071 466 15 Fig 7 Warning and information labels on the Axio Observer Z1 microscope with the LSM 880 scanning module...

Страница 18: ...ICE SAFETY ZEISS Warning and Information Labels LSM 880 16 000000 2071 466 11 2014 V_05 Fig 8 Warning and information labels on the Axio Observer Z1 microscope with the LSM 880 scanning module and Air...

Страница 19: ...R 1 NOTES ON DEVICE SAFETY LSM 880 Warning and Information Labels ZEISS 11 2014 V_05 000000 2071 466 17 Fig 9 Warning and information labels on the Axio Imager Z2 microscope with the LSM 880 scanning...

Страница 20: ...ON DEVICE SAFETY ZEISS Warning and Information Labels LSM 880 18 000000 2071 466 11 2014 V_05 Fig 10 Warning and information labels on the Axio Imager Z2 microscope with the LSM 880 scanning module U...

Страница 21: ...R 1 NOTES ON DEVICE SAFETY LSM 880 Warning and Information Labels ZEISS 11 2014 V_05 000000 2071 466 19 Fig 11 Warning and information labels on the Axio Examiner microscope with the LSM 880 scanning...

Страница 22: ...S ON DEVICE SAFETY ZEISS Warning and Information Labels LSM 880 20 000000 2071 466 11 2014 V_05 Fig 12 Warning and information labels on the Axio Examiner microscope with the LSM 880 scanning module U...

Страница 23: ...ON DEVICE SAFETY LSM 880 Warning and Information Labels ZEISS 11 2014 V_05 000000 2071 466 21 Fig 13 Warning and information labels on the laser and electronic rack Fig 14 Explanatory labels on the l...

Страница 24: ...ER 1 NOTES ON DEVICE SAFETY ZEISS Warning and Information Labels LSM 880 22 000000 2071 466 11 2014 V_05 Fig 15 Warning label on the AOM or attenuator box es Fig 16 Warning label on the motorized peri...

Страница 25: ...rdous radiation exposure The following laser types are currently intended for use in the LSM 880 system The use of any other lasers as the ones listed below is not authorized The values represent the...

Страница 26: ...M system and the Ar laser are equipped with a key interlock Always store keys for laser key switches and if applicable keys for further laser power supply units where they are inaccessible to persons...

Страница 27: ...r the LSM software to switch the reflector turret In case the reflector turret has to be rotated by hand avoid looking into the eyepiece while doing so For the NLO system equipped with a specific push...

Страница 28: ...al workstation with frequent updates of windows patches or security patches The LSM software is released with a specific windows version and patch and this must not be changed unless otherwise release...

Страница 29: ...xture of distilled water and a small quantity of detergent To complete cleaning lightly breathe on the glass surface and rub it dry with a clean cloth Lint or dust is best removed with a clean brush M...

Страница 30: ...e while reducing costly downtime and achieving the best results through the improved performance of your system Choose from service agreements designed to give you a range of options and control level...

Страница 31: ...ing and Dismounting Lamps T PMT and Switching Mirror The ports of the lamps the switching mirror and the transmission T PMT are equipped with hardware interlock devices which have to be operated in th...

Страница 32: ...rew of the port to fix the cap Make sure the pins of the cap depress the sensors of the sensor ring Do not remove the sensor ring from the microscope This might result in failure of laser safety and a...

Страница 33: ...ddrive For changing between the DC out port and NDD T no change of the cabling is necessary When the BiG 2 is moved from the NDD R to the DC out port the following steps have to be performed to change...

Страница 34: ...iG 2 is not attached the laser safety cover must be mounted on the BiG 2 port to close the laser safety loop Push the laser safety cover Fig 21 3 to the port Fig 21 1 and fasten the two fastening scre...

Страница 35: ...ows Loosen the two knurled screws Fig 22 2 Remove the filter insert Fig 22 3 of the BiG 2 Fig 22 1 and put it down How to uninstall a module Disengage the filter module Fig 23 4 from the left spring c...

Страница 36: ...g a P C filter cube with a long pass dichroic projects the longer wavelength range of the emission spectrum to channel 2 and the shorter range of the emission spectrum to channel 1 The assignment of t...

Страница 37: ...manually The module can be exchanged for other objective holders The system needs to be shut down when doing so Mounting the GaAsP NDD 2 channels to the microscope stand Move the rear side of the uni...

Страница 38: ...filter holder Fig 28 3 and slide it out in Reposition the cap and fasten it again with the four screws Do not leave the opening uncovered when working with the system Do not grab into the opening or i...

Страница 39: ...1 5 LSM 880 NLO on Axio Examiner Equipped with NLO Laser and OPO 41 1 6 LSM 880 NLO on Axio Examiner Equipped with Two NLO Lasers 41 1 7 LSM 880 NLO on Axio Observer Equipped with NLO Laser and OPO 4...

Страница 40: ...ts for LSM on small system table measurements in mm The realtime PC and the liquid cooling system are located beneath the system table The Argon laser power supply is placed on top of the laser rack T...

Страница 41: ...stem are located beneath the system table The Argon laser power supply is placed on top of the laser rack The user PC is located under the computer table If present the laser module UV is located on t...

Страница 42: ...f the Argon laser is placed on top of the laser rack The liquid cooling system and the chiller of the NLO laser are located beneath the system table 1 4 LSM 880 Equipped with Two Photon Laser NLO for...

Страница 43: ...of the Argon laser is placed on top of the laser rack The liquid cooling system and the chiller of the NLO laser are located beneath the system table 1 6 LSM 880 NLO on Axio Examiner Equipped with Two...

Страница 44: ...y of the Argon laser is placed on top of the laser rack The liquid cooling system and the chiller of the NLO laser are located beneath the system table 1 8 LSM 880 NLO on Axio Observer Equipped with t...

Страница 45: ...ording to table 2 industrial sector The LSM contains sensitive electronics A few seconds of faulty power can damage parts of the electronics The system is therefore equipped with a surge guard a devic...

Страница 46: ...NLO for Mai Tai Laser or Chameleon 180 150 75 200 Active anti vibration table NLO for two microscope configuration 250 150 75 400 Periscope for two NLO lasers or NLO laser with OPO 40 37 70 40 Active...

Страница 47: ...i Laser or Chameleon 190 160 90 350 Active anti vibration table NLO for two microscope configuration 260 160 90 480 Periscope for two NLO lasers or NLO laser with OPO 70 67 90 60 Active anti vibration...

Страница 48: ...ir conditioning is not directed at the system 2 Operation reduced performance T 15 C to 35 C any conditions different from item 1 and 5 3 Storage less than 16 h T 20 C to 55 C 4 Storage less than 6 h...

Страница 49: ...e laser 405 nm 15 mW Diode laser pulsed cw 405 nm 15 mW cw mode max power ca 15 mW at fiber out range 0 6 15 mW w o attenuator attenuation by a factor of 25 pulsed mode repetition rate 20 50 80 MHz me...

Страница 50: ...CHAPTER 2 SETUP REQUIREMENTS ZEISS System Overview LSM 880 LSM 880 48 000000 2071 466 11 2014 V_05 7 System Overview LSM 880...

Страница 51: ...CHAPTER 2 SETUP REQUIREMENTS LSM 880 System Overview LSM 880 ZEISS 11 2014 V_05 000000 2071 466 49...

Страница 52: ......

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