Center:
When finished with the setup, push
Start experiment
(in the experiments menu) to start
acquire the Z-stack. If Snap is pushed, only a 2D image is acquired at the current location.
Diagram showing the stacks and
first/center/last positions
1.
Go to Live mode, use the
focus knob to find the center
of the sample and push
Center
to select the center
position of the sample
2.
Select
Slice
and give the slice
number for the total range
of the Z-stack. This will be
divided above and below the
center position. Select
Interval and type in the
desired interval between
two stack. If unsure, push
Optimal button to not lose
any information between 2
stacks.
3.
Optimize the pinhole for all
the channels. Note that this
only works for LSM mode.
4.
Set up gradient intensity for
deeper samples (if needed)
