Zeiss LSM 700 Скачать руководство пользователя страница 5 | Manualshive

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Zeiss LSM 700 Скачать руководство пользователя страница 5

p.

5

Sample

mounting

and

viewing

Insert

the

slide

or

dish

to

the

stage

holder.

Push

in

the

Light

switch

between

the

ocular

and

the

confocal.



Via

the

touch

pad:

1.

Choose

the

preferred

objective

2.

Choose

the

proper

filter

set

and

open

the

TL\RL

shutter:

Software

‐

Log

into

BookitLab

and

activate

your

reservation

to

start

Black

Zen

2.3

‐

To

acquire

images

click

Start

System

Filter

set

«
...
3
4
5
6
7
...
»

Содержание LSM 700

Страница 1: ...2 Objectives 2 Filter turret 2 Laser lines 3 Light Path 3 Start up 4 Sample mounting and viewing 5 Software 5 Sample viewing 6 Acquisition 8 LSM Channel mode confocal setting 8 Configuration 8 Acquis...

Страница 2: ...pectral data point Hardware The microscope The LSM 700 is attached to an upright microscope Axio Imager Z2 Objectives Position Objective lens Magnification NA coverslip Working distance Immersion Parf...

Страница 3: ...mission passes through the pinhole aperture and the out of focus light is eliminated 5 6 The variable secondary dichroic beamsplitter VSD is coated with a variable coating that between 420nm 630nm acc...

Страница 4: ...ng order In the power strips turn on switches 1 2 Turn on the stage controller 3 Rotate clockwise the silver key in the lasers electronic unit 4 Turn on the computer and log in User name multilabs Pas...

Страница 5: ...Light switch between the ocular and the confocal Via the touch pad 1 Choose the preferred objective 2 Choose the proper filter set and open the TL RL shutter Software Log into BookitLab and activate...

Страница 6: ...tage to I II or III according to the objective lens see objective lens info on touchpad If you are acquiring transmitted channel set up Kohler illumination 1 Bring the sample into focus 2 Close the fi...

Страница 7: ...p 7 2 5 3 4...

Страница 8: ...ocal Configuration It is highly recommended to seek BCF staff s advice for new fluorophores exact name or new combinations Choose one Load a saved configuration from the Experiment Manager Reuse setti...

Страница 9: ...ThermoFisher Chroma 1 Laser lines 2 Main dichroic mirror 3 PMTs PMT1 and PMT2 4 Variable secondary dichroic beamsplitter VSD 5 Emission Filter selection 6 Emission spectrum with Graphic representatio...

Страница 10: ...logy experiments Frame size 512X512 Speed 8 Averaging Method mean in most cases in low signal with low noise Sum method may be useful Number 1 Mode frame for fixative sample for live cells and in case...

Страница 11: ...lack level black blue pixels in the image o Set the PMT gain to 550 600 o Set the Laser it is recommended to use the lowest as possible and to increase gain master o In case of low signal with high la...

Страница 12: ...ng to the SNR Press Snap in the start buttons Save the image press in the Images and Documents tab right hand side of the window and save in the directory D Users data PI name MM YYYY User name YYYY M...

Страница 13: ...uch as brightness correction over z Time series In the Multidimensional Acquisition options check Time series a new tab Time Series will appear under the Multidimensional Acquisition Section Define nu...

Страница 14: ...mended option the process can be configured in the processing main tool tab In the Acquisition mode tab Frame size Press optimal Zoom It is recommended to set the zoom above 1 2 Scanning speed 7 Avera...

Страница 15: ...After a single scan in the Regions tab choose one of the ROI tools and draw on the acquired image the dimensions can also be set manually in the Width Height textboxes Define which process will be per...

Страница 16: ...before moving to high magnification with small field of view Add a position by pressing Positions below the image container and clicking on the image container that was just acquired at the current st...

Страница 17: ...lose the ZEN software Log off from your account in the BookItlab window Check if another user has a reservation if yes verify his her arrival If you are the last user for the day or the next reservati...

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