Zeiss LSM 700, Инструкция по эксплуатации

Страница: 10 / 17

p. 10
Acquisition & parameter setting
Pull out the lightpath switch between the ocular and the confocal
Parameter setting

Open the Acquisition mode tab

Scan mode : frame (in most cases);
line scan mode can be useful for physiology experiments.

Frame size : 512X512

Speed : 8

Averaging :
Method: mean (in most cases):
in low signal with low noise Sum method may be useful
Number: 1
Mode: frame for fixative sample, for live cells and in case of unstable dye line
mode will be more suitable
Bit Depth: defined the dynamic range of the image, choose 8bit\12 bit according to
the biological question.
Direction: ‐‐‐‐ > (more precise)

Scan area: slight nudge, zoom and rotation