Zeiss LSM 510 Inverted Скачать руководство пользователя страница 10

 

 

 

 

 
 
10  

07/05 

• 

Click on the 

Multi Track 

button in the 

Configuration Control

 window (Fig. 15). 

• 

Click on the 

Descanned

 button if necessary. 

 
The following functions are available in the 

List of

 

Tracks

 panel (Fig. 15). 

Modes 

Switch tracks after each  

Line

 button

 

Tracks are switched during scanning line by line. The following settings 
can be changed between tracks: Laser line and intensity and channels 
incl. settings for gain and offset. 

 
Switch tracks after each  

 

Frame

 button

 

Tracks are switched during scanning frame by frame. The following 
settings can be changed between tracks: Laser line and intensity, all 
filters and beam splitters, the channels incl. settings for gain and offset 
and the pinhole position and diameter. 

Frame Fast

 button

 

The scanning procedure can be made faster. Only and the laser line and 
intensity and the 

Amplifier

 

Offset

 are switched, but no other hardware 

components. The tracks are all matched to the current track with regard 
to emission filter, dichroic beam splitter, setting of Detector Gain, 
pinhole position and diameter. When 

Line

 button is selected, the same 

rules apply as for 

Frame Fast

 

Settings  

Add Track

 button

 

An additional track is added to the configuration list. The maximum of 
four tracks can used. One track each with basic configuration is added, 
i.e.: one Ch 1 channel is activated, all laser lines are switched off, 
emission filters and dichroic beam splitters are set in accordance with the 
configuration last used.  

Remove

 button

 

The single track previously marked in the 

List of Tracks

 panel in the 

Name column is deleted.  

Store/Apply Single Track

 

button

 

Opens the 

Track Configurations

 window. A selected track defined in a 

Channel Mode Configuration can also be stored as a single track for 
single tracking applications. Also, it's possible to load a single track in a 
multi tracking configuration.  

 

A click on this arrow button will move the selected track (highlighted in 
blue) one position upwards in the list box.  

 

A click on this arrow button will move the selected track (highlighted in 
blue) one position downwards in the list box.  

 

• 

Configure each desired track for 

Multi Track

 function as described for 

Single Track

• 

For storing/applying or deleting a 

Channel Mode Configuration

 use the 

Config

 button. 

 

Содержание LSM 510 Inverted

Страница 1: ...LSM 510 and LSM 510 META Laser Scanning Microscopes Brief Operating Manual Release 3 5 July 2005...

Страница 2: ...e LSM 510 and LSM 510 META laser scanning microscope including its original accessories and compatible accessories from other manufacturers may only be used for the purposes and microscopy techniques...

Страница 3: ...nents switch also to ON Now the complete system is ready to be initialized with the LSM Software Switching on the HBO 100 mercury lamp Switch on the HBO 100 mercury lamp via the switch of the power su...

Страница 4: ...10 Switchboard window Clicking on this button activates the complete LSM hardware on line mode Click on the Start Expert Mode button in the LSM 510 Switchboard window The LSM 510 Expert Mode Main menu...

Страница 5: ...ate toolbar Click on the New button in the File subordinate toolbar The Create New Database window appears Select drive C or D from pull down menu Create a new directory if needed Fig 6 Create New Dat...

Страница 6: ...cular tube of the microscope Click on the VIS button to set the microscope for direct observation via the eyepieces of the binocular tube lasers are off Click on the TV button to set the microscope ca...

Страница 7: ...ting the microscope for reflected light Click on the Reflected Light button to open the shutter of the HBO 100 mercury lamp Click on the Reflector button and select the desired filter set by clicking...

Страница 8: ...ick on the Single Track button in the Configuration Control window Fig 12 Click on the Descanned button if necessary The Beam Path and Channel Assignment panel of the Configuration Control window disp...

Страница 9: ...he first line of the Configurations list box and click an Store For loading an existing configuration select it in the list box and click on Apply For deleting an existing configuration select it in t...

Страница 10: ...position and diameter When Line button is selected the same rules apply as for Frame Fast Settings Add Track button An additional track is added to the configuration list The maximum of four tracks c...

Страница 11: ...lts in the best signal to noise ratio Scan speed 8 usually produces good results Us speed 6 or 7 for superior images Choosing the dynamic range Select the dynamic range 8 or 12 Bit per pixel in the Pi...

Страница 12: ...same Optical Size This is important for colocalization studies Image acquisition Once you have set up your parameter as defined in the above section you can acquire a frame image of your specimen Use...

Страница 13: ...gh it appears blue on the screen Blue zero minimum Adjusting the laser intensity Set the Pinhole to 1 Airy Unit Fig 21 Set the Detector Gain high When the image is saturated reduce AOTF transmission i...

Страница 14: ...upper position of the specimen area where the Z Stack is to start Click on the Mark First button to set the upper position of the Z Stack Then focus on the lower specimen area where the recording of t...

Страница 15: ...ing off the system Click on the File button in the Main menu and then click on the Exit button to leave LSM 5 software program Fig 5 If any lasers are still running you should shut them off now in the...

Страница 16: ......

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