OPERATION
Axio Observer
Illumination and contrast techniques
ZEISS
12/2016
431004-7244-001
147
•
Set the light intensity to a comfortable level by using the illumination control wheel (Fig. 148/
32
).
•
Close the luminous-field diaphragm
(Fig. 148/
10
) until it is visible in the field of view
(not necessarily in focus) (Fig. 149/
A
).
•
Bring the edge of the luminous-field diaphragm
into focus (Fig. 149/
B
) by adjusting the height
of the condenser (Fig. 148/
13
).
•
Center (Fig. 149/
C
) the luminous-field
diaphragm using the centering screws
(Fig. 148/
8
) and open until the edge of the
diaphragm just disappears from the field of
view (Fig. 149/
D
).
•
To set the aperture diaphragm, remove one eyepiece from the eyepiece tube and adjust the aperture
diaphragm of the condenser to approximately 2/3 the diameter of the exit pupil of the eyepiece
(Fig. 149/
E
).
The settings required for optimum contrast depend on the specimen.
•
Reinsert the eyepiece and where necessary refocus on the specimen using the fine focusing drive.
•
Adjust the light intensity using the rocker switch.
The field size and objective aperture change each time the objective is changed, so that for
optimal results the luminous-field diaphragm and aperture diaphragm should be readjusted
whenever the objective is changed.
Fig. 149
Diaphragm settings in transmitted
light brightfield acc. to KÖHLER
Содержание Axio Observer Series
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