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OPERATION

Photomicrography with

Carl Zeiss

Color balance

35 mm SLR cameras

3-14

B 40-046 e 03/99

3.2.8.4

Computing the Magnification

−

SLR connections, 2.5

/ T2 adapters:

The magnification of the image on the 35 mm film M

film

is the product of objective magnification M

obj

and the factor of the T2 adapter 2.5

film

= M

obj

2.5

−

Objective f' = 63 mm with photo eyepieces M

eyep.

, = 10

The magnification of the image on the 35 mm film M

film

is calculated from the objective magnification

obj

,, the focal length of objective f

’ = 63 mm and the reference distance of vision l = 250 mm

obj

eyep.

’

film



obj

63 mm

Film



250 mm

film

= M

obj

2.5

−

If an intermediate imaging system is also used, e.g. an Optovar or zoom system, the factor of the M

intermediate imaging optics also plays a part in the magnification:

film

= M

obj

2.5

3.2.8.5

Further Filters

In addition to the conversion, gray and neutral-density filters listed on page 3-11, the following filters are
also available for photomicrography:

Filter

d = 32 mm

d = 25 mm

Conversion filter CB 6

467851-0000-000

Conversion filter CB 3

467852-0000-000

Interference green filter

467803-0000-000

447805-9901-000

Содержание 35 mm SLR Camera

Страница 1: ...Photomicrography with 35 mm SLR Cameras Operating Manual ...

Страница 2: ...change the manual is not covered by an update service Unless expressly authorized forwarding and duplication of this document and the software package and the utilization and communication of its contents are not permitted Violations will entail an obligation to pay compensation All rights reserved in the event of granting of patents or registration of a utility model Issued by Carl Zeiss Microsco...

Страница 3: ... SLR Camera on 2 5 T2 Camera Adapter on the Front Port of the Axiovert 25 C 0 9 Overall View of 35 mm SLR Camera above 63 mm Objective and Connector for Microscope Camera Image Position 44 mm with 10 Photo Eyepiece on the Axioskop with 44 mm Camera Port 0 10 CHAPTER 1 INSTRUMENT DESCRIPTION 1 1 1 Instrument Description 1 3 1 1 Name and Intended Application 1 3 1 2 Instrument Description and Main F...

Страница 4: ...Cameras to Front Camera Port of Axiovert 35 35 M 135 135 M 2 8 2 3 5 Attachment of 35 mm SLR Cameras with Objective f 63 mm to Camera Ports with 40 mm Clamping Diameter 2 9 2 4 Determination of the Exposure Time Range for unblurred Photographs 2 10 CHAPTER 3 OPERATION 3 1 3 OPERATION 3 1 3 1 Adaptation and Functions 3 2 3 2 Photomicrography with 35 mm SLR Cameras 3 3 3 2 1 Photography in a few Ste...

Страница 5: ...nance Care Troubleshooting and Service 4 2 4 1 Instrument Care 4 2 4 2 Instrument Maintenance 4 3 4 2 1 Performing Checks 4 3 4 2 2 Changing the Batteries 4 3 4 3 Troubleshooting 4 3 4 4 Consumables and Tools 4 4 4 5 Requesting Service 4 4 ANNEX A 1 List of Abbreviations A 2 Physical and Technical Units A 3 List of Key Words A 4 EC Declaration of Conformity A 7 ...

Страница 6: ...or any other applications possibly also involving individual modules or single parts This also applies to all service or repair work which is not carried out by authorized service personnel Furthermore this forfeits all the claims against warranty Dust and dirt can impair the performance of the instrument Therefore the instrument must be protected against these influences as far as possible and co...

Страница 7: ...d by natural wear wearing parts in particular and improper use The instrument manufacturer is not liable for damage caused by faulty operation negligence or any other meddling with the instrument particularly the removal or replacement of instrument components or the use of accessories from other manufacturers This forfeits all the claims against warranty With the exception of the work specified i...

Страница 8: ... Overall View of 35 mm SLR Camera on 2 5 T2 Adapter for SLR Cameras on 60 mm Camera Port of the Stemi 2000 C Stereomicroscope in this Example 1 Cable release 2 Eyepiece cover 3 35 mm SLR camera 4 T2 adapter 5 2 5 T2 adapter for SLR camera 6 60 mm camera port 7 Stemi 2000 C microscope body 8 Eyepiece ...

Страница 9: ...iew Carl Zeiss B 40 046 e 03 99 0 9 Overall View of 35 mm SLR Camera on 2 5 T2 Adapter on Front Camera Port of the Axiovert 25 C 1 front camera port 2 2 5 T2 camera adapter 3 T2 adapter 4 35 mm SLR camera 5 Eyepiece cover 6 Cable release 7 Eyepiece 8 Axiovert 25 C ...

Страница 10: ...bjective and Adapter for Microscope Camera Image Position 44 mm with 10 Camera Eyepiece on 44 mm Camera Port i e on the Axioskop with Phototube in this Example 1 Cable release 2 Eyepiece cover 3 35 mm SLR camera 4 T2 adapter 5 63 mm objective 6 Adapter for microscope camera image position 44 mm with 10 phototube 7 44 mm camera port 8 Axioskop ...

Страница 11: ...ents 1 INSTRUMENT DESCRIPTION 1 3 1 1 Name and Intended Application 1 3 1 2 Instrument Description and Main Features 1 3 1 3 35 mm SLR Camera Components 1 4 1 4 Overview of Image Positions Tube Adapters relevant Phototubes and Microscope Stands 1 7 1 5 Technical Data 1 9 List of illustrations Fig 1 1 35 mm SLR camera components 1 4 ...

Страница 12: ...INSTRUMENT DESCRIPTION Photomicrography with Carl Zeiss 35 mm SLR cameras 1 2 B 40 046 e 03 99 ...

Страница 13: ...in the eyepiece intermediate image The instrument side connection surfaces of the adapters correspond to the camera ports of the microscopes and phototubes from Carl Zeiss while the T2 adapter is available on the camera side The T2 adapter is standardized M 42 0 75 thread distance between T2 contact surface and film plane 54 9 mm SLR cameras are screwed on the T2 connector via the T2 adapter suita...

Страница 14: ...INSTRUMENT DESCRIPTION Photomicrography with Carl Zeiss Components 35 mm SLR cameras 1 4 B 40 046 e 03 99 1 3 35 mm SLR Camera Components Fig 1 1 35 mm SLR camera components ...

Страница 15: ... adapter comp 6 2 5 T2 camera adapters 8 11 Photo eyepiece 9 Adapter for microscope camera image position 60 mm 10 Adapter for microscope camera image position 44 mm 12 Camera port clamping diameter 40 mm 13 Camera port image position 44 mm 14 Camera port image position 60 mm 16 Contact surface of Axiovert 25 C CFL CA front port 17 Camera connection to front port of Axiovert 35 35 M 135 135 M 18 M...

Страница 16: ...T2 adapter for Minolta Autofocus Minolta Dynax 416012 0000 000 T2 adapter for Canon Autofocus Canon EOS 416013 0000 000 T2 adapter for Praktica L M 42 1 thread 263060 2505 124 Adapter bayonet M 42 1 thread 000000 0225 954 T2 Adapter for Praktica B bayonet consisting of T2 Adapter for Praktica L M 42 1 thread and adapter bayonet M 42 1 thread T2 camera adapters 4 Adapter for 2 5 SLR camera for T2 4...

Страница 17: ...000 452970 0000 000 452974 0000 000 450960 0000 000 450962 0000 000 450963 0000 000 450964 0000 000 Axiolab Axiolab A Axiolab Pol 455005 0000 000 Stemi DRC 455080 0000 000 455081 0000 000 455082 0000 000 Stemi SV 6 SV 11 SV 11 Apo 455053 0000 000 455055 0000 000 Stemi 2000 C 2000 CS 452934 0000 000 Axiotech Axiotech vario 451321 0000 000 451322 0000 000 451324 0000 000 451325 0000 000 Axiovert 100...

Страница 18: ...M 452920 0000 000 452921 0000 000 452923 0000 000 452925 0000 000 452926 0000 000 452930 0000 000 452931 0000 000 452932 0000 000 Axioplan Axiotron Axiotron 2 452146 0000 000 Axioplan2 Clamping diameter 40 mm 452903 0000 000 Standard 20 25 451474 0000 000 Co observation equipment 473024 0000 000 Universal Photomicroscope 475083 0000 000 Stemi SR SV 8 475084 0000 000 Stemi DRC old Special adapter T...

Страница 19: ...ective f 63 mm in T2 mount with clamping ring for D 40 mm approx 56 107 mm Photo eyepiece S Pl 10 20 approx 28 60 mm Wide angle eyepiece Kpl 10 20 Br approx 25 60 mm Monocular telescope 3 12 B approx 28 62 mm Photo reticle MC 2 5 approx 26 21 19 2 mm Weights 35 mm SLR camera housing Contax 167 MT 0 620 kg 35 mm SLR camera housing Contax Aria 0 460 kg 35 mm SLR camera housing Ricoh XR X 3000 0 480 ...

Страница 20: ...20 1 battery KS 28 6V or 4 batteries LR 20 1 5 V Ambient conditions Storage and transport in packaging Permissible ambient temperature 10 to 60 C Permissible relative humidity 20 to 85 at 35 C Atmospheric pressure 800 hPa to 1060 hPa Operation Permissible ambient temperature 10 to 45 C Permissible relative humidity 20 to 85 at 35 C Permissible altitude for operation max 2000 m Atmospheric pressure...

Страница 21: ...dapter to Front Port of Axiovert 25 C CFL CA 2 7 2 3 4 Attachment of 35 mm SLR Cameras to Front Camera Port of Axiovert 35 35 M 135 135 M 2 8 2 3 5 Attachment of 35 mm SLR Cameras with Objective f 63 mm to Camera Ports with 40 mm Clamping Diameter 2 9 2 4 Determination of the Exposure Time Range for Unblurred Photographs 2 10 List of illustrations Fig 2 1 Photo reticle MC 2 5 2 2 Fig 2 2 Attachmen...

Страница 22: ...nts from Carl Zeiss are delivered in separate packaging Remove all components from the packaging and use the delivery note to check for completeness Properly dispose of original packaging or keep it for storage or return of the instrument to the manufacturer 2 2 Insertion of Photo Reticles MC 2 5 2 2 1 Assignment of Photo Reticles MC 2 5 The photo reticle shows the boundaries of the image section ...

Страница 23: ... 9902 000 455042 0000 000 000000 1026 548 455043 0000 000 455044 0000 000 000000 1016 758 444235 0000 000 444034 0000 000 444234 0000 000 MC 2 5 d 21 mm 454025 0000 000 Kpl 10 20 Br foc E PL 10 20 Br foc PL 16 16 Br foc W PL 16 16 Br foc W 25 10 foc 464048 9902 000 444232 0000 000 444236 0000 000 444054 0000 000 455048 0000 000 455046 0000 000 MC 2 5 d 19 mm 476021 0000 000 KF 10 18 Br foc Kpl 10 ...

Страница 24: ... 2 W but by the red dot 2 2 R For focusing eyepieces without photo reticle set diopter zero position to the white dot 2 2 W and for focusing eyepieces with photo reticle set diopter zero position to the red dot 2 2 R If photo reticles are inserted into the unscrewed mount by the customer attention must be paid to the labeling being visible the right way up after insertion 2 2 3 Focusing of Eyepiec...

Страница 25: ...LR camera housing 2 3 2 Remove dust cover from 2 5 T2 adapter for SLR cameras 2 3 4 or SLR adapter 2 5 comp 2 3 5 Screw thread of 2 5 T2 adapter for SLR cameras 2 3 4 or of SLR adapter 2 5 comp 2 3 5 in the T2 adapter 2 3 3 attached to the SLR camera housing 2 3 2 Remove dust cover from phototube 2 3 6 Insert 2 5 T2 adapter for SLR cameras 2 3 4 or SLR adapter 2 5 comp 2 3 5 with SLR camera housin...

Страница 26: ...mera with image position 44 mm 2 4 7 in the 44 mm camera port 2 4 8 or the connector for microscope camera with image position 60 mm 2 4 10 in the 60 mm camera port 2 4 11 Tighten clamping screws 2 4 9 12 to the 44 or 60 mm camera ports using the supplied ball headed screwdriver 2 4 13 Insert photo eyepiece S Pl 10 20 2 4 6 in the connectors for microscope cameras 2 4 7 10 Attach objective f 63 mm...

Страница 27: ...er for Axiovert 25 2 5 4 Screw thread of 2 5 T2 adapter for Axiovert 2 5 4 in the T2 adapter 2 5 3 attached to the SLR camera housing 2 5 2 Remove dust cover from the front camera port of the Axiovert 25 2 5 6 Push 2 5 T2 camera adapter for Axiovert 25 2 5 4 with SLR camera housing in the front camera port of the Axiovert 25 2 5 6 until stop Align SLR camera horizontally and tighten clamping screw...

Страница 28: ...overt 35 35 M 135 135 M Attach the SLR camera housing 2 6 2 to the relevant T2 adapter 2 6 3 If required align camera horizontally loosen three set screws 2 6 4 on the T2 adapter 2 6 3 using watchmaker s screwdriver align camera 2 6 2 horizontally and tighten set screws 2 6 4 again using the watchmaker s screwdriver Attach cable release 2 5 1 if required If the T2 adapter for the Contax bayonet 2 ...

Страница 29: ... Remove dust covers from objective f 63 mm 2 7 4 Screw thread of objective f 63 mm 2 7 4 in the T2 adapter 2 7 3 attached to the SLR camera housing 2 7 2 Remove dust cover from the camera port with 40 mm clamping diameter 2 7 7 Insert wide angle eyepiece Kpl 10 20 Br 2 7 6 in camera adapter 2 7 7 Attach objective f 63 mm 2 7 4 with SLR camera housing to adapter 2 7 7 containing the photo eyepiece ...

Страница 30: ...e object Set brightfield illuminator according to KÖHLER Make an exposure series trigger one photograph each with the exposure times 1 250s 1 125s 1 60s 1 30s 1 15s 1 8s 1 4s 1 2s and 1s in the Automatic mode The different exposure times must be set by adjusting the lamp voltage and if required by insertion of neutral density filters into the illumination beam path Make a second exposure series us...

Страница 31: ...opy Technique 3 5 3 2 5 Setting and Focusing of Image Section 3 6 3 2 6 Exposure Functions 3 7 3 2 7 Trigger the Exposure 3 10 3 2 8 Further Notes 3 11 3 2 8 1 Avoid Blurs 3 11 3 2 8 2 Color Balance in Color Reversal Films 3 11 3 2 8 3 Darkfield and Fluorescence Photography 3 13 3 2 8 4 Computing the Magnification 3 14 3 2 8 5 Further Filters 3 14 3 3 Using Digital Cameras 3 15 List of illustratio...

Страница 32: ...rams film insertion are described in the operating instructions of the relevant SLR camera The T2 adapters suitable for the used 35 mm SLR cameras are attached and removed in the same way as camera lenses This is also described in the relevant camera instructions Further information on the use of 35 mm SLR cameras for photomicrography are given in section 3 2 photomicrography with SLR cameras The ...

Страница 33: ...n 3 2 2 setting of color temperature and image brightness Insert 135 film 35 mm in 35 mm SLR camera also see section 3 2 3 selecting a film Set exposure correction for illumination technique also see section 3 2 4 exposure correction depending on microscopy technique If a suitable data back is used with the 35 mm SLR camera enter the data also see the operating instructions of the used data back C...

Страница 34: ...are used for color exposures In general reversal films for artificial light 3200 K are recommended If daylight film is used a conversion filter 3200 5500 K is required Conversion filter 3200 5500 K d 32 mm 467847 0000 000 d 25 mm 447825 000 000 d 18 mm 467854 0000 000 Films labeled Professional feature closer tolerances for speed and color balance i e exposure and color are more homogenous DX code...

Страница 35: ...ive tape Avoid overly long film as some cartridges cannot be used for 36 frames or for every film This could damage the film advance mechanism in the film cassette 3 2 4 Exposure Correction Depending on the Microscopy Technique Exposure corrections may be required depending on which microscopy technique is used The following values are standard in photomicrography Microscopy technique Correction v...

Страница 36: ...rd with 1 EV For the setting of exposure correction and exposure bracketing please see the operating instructions of the used 35 mm SLR camera 3 2 5 Setting and Focusing of Image Section Via binocular tube the section of the microscope object set in accordance with the photo reticle is imaged on the film in focus when the focusing cross and the specimen are clearly defined simultaneously To achiev...

Страница 37: ...glass spot with reticle are ideal for the setting and focusing of the image section Some camera manufacturers offer such a changeable screen 3 2 6 Exposure Functions 35 mm SLR cameras with TTL Through The Lens metering These 35 mm SLR cameras permit the following metering modes center weighted averaging spot metering and multiple area metering Each of these metering modes permits selection between...

Страница 38: ... aperture 2 8 is 8s 4s for aperture 2 and only 2 s for aperture 1 4 35 mm SLR cameras for photomicrography Center weighted averaging spot metering and multiple area metering can also be used for photomicrography with 35 mm SLR cameras For photomicrography with 35 mm SLR cameras T2 adapters are attached to the camera housings with TTL metering instead of the camera lens Aperture functions are not t...

Страница 39: ... 4 Exposure after manual exposure time alignment with preselected open aperture depending on the image brightness the shutter speed is matched for the open aperture i e aperture 1 2 or 1 4 by turning the shutter speed knob until zero alignment of an indicator e g pointer on indicator Exposure after manually set exposure time and B function The measuring range of cameras with T2 adapter is determin...

Страница 40: ... exposure time to be controlled Therefore you should work in a darkened room and after reading or aligning the exposure time in the viewfinder you should always cover the viewfinder with the eyepiece cover for SLR before the automatic exposure is triggered For the setting of the various measuring techniques and exposure modes and for the display of exposure times please see the operating manual of...

Страница 41: ... 12 transmission 467841 0000 000 gray filter 0 03 3 transmission 467842 0000 000 neutral density filters 0 25 25 transmission 447836 0000 000 467856 0000 000 neutral density filters 0 06 6 transmission 447835 0000 000 467855 0000 000 The exposure time for the exposure to be taken can be read off the outside of the camera housing display or shutter speed setting knob or from the viewfinder display ...

Страница 42: ...ray and white Determine the Correction Filter Place filter of complementary color of the color distortion on the slide to be corrected Color distortion Color of the CC filter Blue Yellow Y Green Magenta M Red Bluish green Cyan C Yellow Blue B Magenta Green G Bluish green Red R If for example a filter of density 10 does not display the required color balance on viewing you should use a CC filter of...

Страница 43: ...use fast films Granularity is hardly ever a problem in fluorescence micrographs The dark or black background often accounts for a substantial part of the area used for automatic exposure metering Estimate the ratio of bright and dark areas and correct the exposure time accordingly Because of the high contrast the exposure latitude is rather high since luminous structures are always well set off ag...

Страница 44: ...n Mobj the focal length of objective fOb 63 mm and the reference distance of vision l 250 mm Mobj Meyep fOb Mfilm l Mobj 10 63 mm MFilm 250 mm Mfilm Mobj 2 5 If an intermediate imaging system is also used e g an Optovar or zoom system the factor of the MZ intermediate imaging optics also plays a part in the magnification Mfilm Mobj MZ 2 5 3 2 8 5 Further Filters In addition to the conversion gray ...

Страница 45: ...l resolution The motion of the sensor array can cause vibrations resulting in blurred photos Cameras with area sensor e g Kodak EOS DCS 1 Canon EOS 1 camera housing Kodak DCS 460 Nikon N90s camera housing The outer dimensions of the sensor chip i e 18 4 mm x 27 6 mm determine the image contents of the digital photo Here the image contents totals approx of the photo on 35 mm film Exposure is made v...

Страница 46: ...OPERATION Photomicrography with Carl Zeiss Color balance 35 mm SLR cameras 3 16 B 40 046 e 03 99 ...

Страница 47: ...e 03 99 4 1 MAINTENANCE CARE TROUBLESHOOTING AND SERVICE Contents 4 MAINTENENANCE CARE TROUBLESHOOTING AND SERVICE 4 2 4 1 Instrument Care 4 2 4 2 Instrument Maintenance 4 3 4 2 1 Performing Checks 4 3 4 2 2 Changing the Batteries 4 3 4 3 Troubleshooting 4 3 4 4 Consumables and Tools 4 4 4 5 Requesting Service 4 4 ...

Страница 48: ...85 isopropanol and 15 medical alcohol benzoline clean glass surface using a slightly moistened wad of cotton by moving in circles starting in the middle Remove dust from optical surfaces using a natural hair brush or an air blower Please also see the additional notes on camera maintenance contained in the operating manual of the used SLR camera When using the components in humid climatic zones pro...

Страница 49: ...er sufficient this is indicated in the SLR camera e g by flashing of the display If any damage is visible switch off the SLR camera Repairs may only be performed by authorized service staff therefore please contact the Carl Zeiss service department see page 4 4 4 2 2 Changing the Batteries See the relevant notes in the manual supplied by the camera manufacturer 4 3 Troubleshooting See the relevant...

Страница 50: ... of mechanical optical or electronic components of the 35 mm SLR camera may only be performed by Carl Zeiss service staff or specially authorized personnel To ensure the optimum setting and trouble free function of the components even for a longer period of time we would recommend you to conclude a service maintenance contract with Carl Zeiss Should any components have to be ordered subsequently o...

Страница 51: ...otomicrography with ANNEX 35 mm SLR cameras Contents Carl Zeiss B 40 046 e 03 99 A 1 ANNEX List of Abbreviations A 2 Physical and Technical Units A 3 List of Key Words A 4 EC Declaration of Conformity A 5 ...

Страница 52: ...iation DX coding system for the storage of electronically legible information e g film speed E PL Extra Plan eyepiece model EV Exposure Value of light f 1 4 camera lens with focal length f with aperture ratio 1 1 4 f focal length on the image side f Obj focal length of objective FL fluorescence foc focusing G green ICS Infinity Color Corrected System IM Inverted Microscope ISO International Organi...

Страница 53: ...ins open after first press and closes only after second press see B function Tmax maximum exposure time Tmin minimum exposure time VObj magnification of the objective Veyep magnification of the eyepiece VZ intermediate magnification W PL Weitfeld Plan wide angle eyepiece model Y Yellow Physical and Technical Data C Centigrade hPa Hecto Pascal K Kelvin kg kilogram m meter mm millimeter s second V V...

Страница 54: ...e 3 11 color distortion 3 12 color reversal film 3 11 color temperature 3 3 color compensating CC filters 3 11 consumables 4 4 D darkfield 3 13 dimensions 1 9 double line cross 2 2 E exposure correction 3 5 F film cassette 3 3 I insert photo reticles 2 2 Instrument care 4 2 instrument maintenance 4 3 M maintenance 4 2 4 3 O operation 1 10 P performing checks 4 3 phototube 2 5 2 9 S START UP 2 1 T ...

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