41
Calibration
Chlorophyll
Phycocyanin
Phycoerythrin
Temp (°C)
RFU
μ
g/L
RFU
μ
g/L
RFU
μ
g/L
30
14.0
56.5
11.4
11.4
37.3
104.0
28
14.6
58.7
13.1
13.1
39.1
109.0
26
15.2
61.3
14.1
14.1
41.0
115.0
24
15.8
63.5
15.0
15.0
43.0
120.0
22
16.4
66
16.0
16.0
45.0
126.0
20
17.0
68.4
17.1
17.1
47.0
132.0
18
17.6
70.8
17.5
17.5
49.2
138.0
16
18.3
73.5
19.1
19.1
51.4
144.0
14
18.9
76
20.1
20.1
53.6
150.0
12
19.5
78.6
21.2
21.2
55.9
157.0
10
20.2
81.2
22.2
22.2
58.2
163.0
8
20.8
83.8
22.6
22.6
60.6
170.0
TAL Calibration
A 1- or 2-point calibration can be completed for all channels on the TAL-PC and TAL-PE sensors.
A 1-point calibration, typically completed in clear deionized or distilled water, is simply a re-zeroing of the sensor.
This calibration does not reset the second point entered during the previous 2-point calibration. The consequence is
that error will be alleviated at and near zero, but more error can accumulate in the measurement the farther away from
zero the measured value is. The amount of error is dependent upon how much the second point drifts, which is not
always equivalent to how much the zero point drifts.
For many users, especially those with sites where pigment is rarely detected and values are at or near zero most of the
time, the far-from-zero accumulation of error is a non-issue. For others, it is best to perform a 2-point calibration using a
Rhodamine WT solution.
Rhodamine WT Dye Solution Preparation
(continued)