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DO Calibration

The Pro20/Pro20i can be calibrated in % saturation, mg/L, or ppm.  Calibration of any 
option (%, mg/L, or ppm) will automatically calibrate the others.  The Pro20/Pro20i can 
be calibrated with the press of one key when One Touch Cal is enabled in the System 
Setup menu.  

The following procedure outlines the % saturation calibration option with and without 
One Touch Cal enabled.

1. 

Moisten the sponge in the cal/transport sleeve with a small amount of water 
and install it on the probe.  The cal/transport sleeve ensures venting to the 
atmosphere.  

Make sure the DO and temperature sensors are not immersed 

in the water.

2. 

Turn the instrument on.  If using a polarographic sensor, wait 10 minutes for the 
DO sensor to stabilize.  Galvanic sensors do not require a warm up time.

3. 

Ensure the barometer reading and salinity correction values along the bottom of 
the screen are accurate.

4. 

Press and hold the 

Cal

 key for three seconds.

5. 

If One Touch Cal is enabled, the instrument will indicate 

Calibrating %DO

 on 

the display and automatically calibrate the sensor to the barometer reading and 
salinity correction value.  

6. 

If One Touch Cal is not enabled, highlight 

%

 or 

%Local

 and press 

Enter

.   The 

Pro20/Pro20i will display the current DO% and temperature readings along 
with the % calibration value.  Wait at least 3 seconds, then, once the DO% and 
temperature readings are stable, press 

Enter

 to complete the calibration. 

7. 

Calibration Successful

 will display for a few seconds to indicate a successful 

calibration and then the instrument will return to the run screen. 

8. 

If the calibration is unsuccessful, an error message will display on the screen.  
Press the 

Cal

 key to exit the error message and return to the run screen.

Salinity Compensation Calibration

The Pro20/Pro20i uses an inputted salinity value in ppt (parts per thousand) to 
compensate dissolved oxygen mg/L values. 

2. 

From the run screen, use the up or down arrow keys to highlight the barometer 
box then press enter.  

3. 

Use the up or down arrow keys to adjust the barometer reading to the 

local, 

true barometric pressure

4. 

Press enter to confirm and save the barometer adjustment.

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