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Tuning in APCI Mode

Specify these suggested parameters in the APCI+ Source page fields.

Select all four Peak Editor fields, and enter these mass assignment values.

Start the LC pump, specifying a 1 mL/min flow rate.

Click

(Syringe pump) to infuse the startup solution into the source.

Click the

Analyser

tab, and ensure the syringe pump flow rate is 10 µL/min.

Turn the probe adjustor (see

Figure 2-8

) in either direction to optimize peak

intensities.

Parameter

Suggested Value

Corona (µA)

0.1

Cone (V)

Extractor (V)

RF Lens (V)

0.0

Source Temp (

120

Desolvation Gas Flow (L/hr)

300

APcI Probe Temp (

500

Cone Gas Flow (L/hr)

STOP

Attention:

Before proceeding, let the source temperature, desolvation gas

flow, desolvation temperature, and cone gas flow reach their setpoints. See

Section 3.4

for information about monitoring readbacks.

Mass

Span

Gain

175.1

609.3

1080.8

2034.6

Содержание Micromass ZQ 2000

Страница 1: ...Waters Micromass ZQ Detector Operator s Guide 34 Maple Street Milford MA 01757 71500044702 Revision B...

Страница 2: ...sequential damages in connection with or arising from the use of this document 2002 WATERS CORPORATION PRINTED IN THE UNITED STATES OF AMERICA ALL RIGHTS RESERVED THIS DOCUMENT OR PARTS THEREOF MAY NO...

Страница 3: ...ould void the user s authority to operate the equipment Important Toute modification sur cette unit n ayant pas t express ment approuv e par l autorit responsable de la conformit la r glementation peu...

Страница 4: ...trouvant proximit N utilisez pas de tuyau de Tefzel fortement ab m ou d form N utilisez pas de tuyau de Tefzel avec de l acide sulfurique ou nitrique ou du t trahydrofurane THF Sachez que le chlorure...

Страница 5: ...lsolfossido provocano rigonfiamento nei tubi Tefzel che riducono notevolmente il limite di pressione di rottura dei tubi stessi Advertencia manipular con precauci n los tubos de pol mero bajo presi n...

Страница 6: ......

Страница 7: ...d fectueuses Vorsicht Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgem er Verwenddung des Ger tes unter Umst nden nicht ordnungsgem funktionieren Precauzione l utente deve essere al corr...

Страница 8: ...d viter tout risque d incendie Vorsicht Zum Schutz gegen Feuergefahr die Sicherungen nur mit Sicherungen des gleichen Typs und Nennwertes ersetzen Precauzione per una buona protezione contro i rischi...

Страница 9: ...d effectuer la maintenance de l instrument Vorsicht Zur Vermeidung von Stromschl gen sollte das Ger t vor der Wartung vom Netz getrennt werden Precauzione per evitare il rischio di scossa elettrica sc...

Страница 10: ...continua Corriente continua Alternating current Courant alternatif Wechselstrom Corrente alternata Corriente alterna Protective conductor terminal Borne du conducteur de protection Schutzleiteranschl...

Страница 11: ...au guide Vorsicht oder lesen Sie das Handbuch Prestare attenzione o fare riferimento alla guida Act e con precauci n o consulte la gu a Caution hot surface or high temperature Attention surface chaud...

Страница 12: ...ica alta tensi n Caution risk of needle stick puncture Attention risques de perforation de la taille d une aiguille Vorsicht Gefahr einer Spritzenpunktierung Precauzione rischio di puntura con ago Pre...

Страница 13: ...on Sous tension Netzschalter ein Alimentazione elettrica attivata Alimentaci n el ctrica conectada Electrical power off Hors tension Netzschalter aus Alimentazione elettrica disattivata Alimentaci n e...

Страница 14: ...dling CDC NIH Manual 1984 Calibration Follow the calibration methods set forth in this guide using pure standards The concentration range should cover the entire range of quality control samples typic...

Страница 15: ...Requirements 31 2 2 Unpacking and Inspecting 32 2 3 Installing the Detector 33 2 3 1 Installing the Rotary Pump 33 2 3 2 Installing the Oil Return Connection Kit 34 2 3 3 Connecting the Nitrogen Suppl...

Страница 16: ...tion 48 3 3 2 Installing the Tee Fitting 49 3 3 3 Specifying Parameters on the APCI Source Page 49 3 4 Readbacks 52 Chapter 4 Calibrating 54 4 1 Setting Up the Calibration File 54 4 2 Setting Calibrat...

Страница 17: ...leaning the APCI Probe Tip 91 5 2 7 Cleaning and Replacing the Corona Discharge Needle 91 5 3 Replacing Parts 92 5 3 1 Replacing the Ion Block Cartridge Heater 92 5 3 2 Replacing the Stainless Steel C...

Страница 18: ...lling the Corona Discharge Needle 108 A 1 2 Setting Up MassLynx 110 A 2 Daidzein Test 117 A 2 1 Test Conditions 118 A 2 2 Signal to Noise Ratio 118 Appendix B Specifications 119 B 1 ZQ Detector Specif...

Страница 19: ...log Box 45 3 5 Tune Window Displaying the Analyser Page 47 3 6 Combined Flow into the Tee 49 3 7 Tune Window Displaying the APCI Source Page 50 3 8 Readbacks Dialog Box 52 3 9 Diagnostics Page Readbac...

Страница 20: ...ck Rear View 88 5 13 Hexapole Assembly 89 5 14 Ion Block Cartridge Heater 92 5 15 Replacing the Heater Cartridge 93 5 16 ESI Probe Tip with Capillary Protruding 0 5 mm 95 A 1 ZQ Mass Detector Front Vi...

Страница 21: ...9 5 1 Maintenance Schedule 74 6 1 Hardware Troubleshooting 101 A 1 HPLC Conditions 118 A 2 MS Conditions 118 B 1 ZQ Detector Operational Specifications 119 B 2 ZQ Detector Environmental Specifications...

Страница 22: ...List of Tables 22 C 7 Source and Analyser Components 126 C 8 Washers Screws and Nuts 127 C 9 Miscellaneous Components 128...

Страница 23: ...tart it operating and tune and calibrate it Chapter 4 describes how to set up a calibration file and specify calibration parameters in MassLynx Chapter 5 explains routine maintenance procedures Chapte...

Страница 24: ...ile Print For optimum print quantity Waters recommends that you specify a PostScript printer driver for your printer Ideally use a printer that supports 600 dpi print resolution Documentation Conventi...

Страница 25: ...le Monitoring Readbacks are described in Section 3 4 Readbacks Keys The word key refers to a computer key on the keypad or keyboard Screen keys refer to the keys on the instrument located immediately...

Страница 26: ...burns turn off the lamp at least 30 minutes before removing it for replacement or adjustment Caution To avoid electrical shock and injury unplug the power cord before performing maintenance procedure...

Страница 27: ...lvation The ions ultimately reach the quadrupole which separates them according to their mass to charge ratios A photomultiplier then detects the mass separated ions amplifies their signals and sends...

Страница 28: ...rkstation uses a Windows NT Windows 2000 or Windows XP color graphical environment and allows full user interaction with the keyboard or mouse A network link communicates between the workstation and t...

Страница 29: ...ionization occurs by means of a proton transfer mechanism For negative ions the mechanism is proton abstraction 1 3 MassLynx 4 0 Software MassLynx 4 0 software permits these major operations Configuri...

Страница 30: ...arizes these procedures Figure 2 1 Installing the Detector Installation Begins Installation Complete Select and Prepare Appropriate Site Unpack and Inspect Install ESI Probe Connect Rheodyne Injector...

Страница 31: ...o allow for service access Note Movable equipment can be located as close as 4 75 in 120 mm Left side 0 0 in mm Top 11 in 28 cm Power requirements Grounded AC 230 V 50 60 Hz Electromagnetic fields No...

Страница 32: ...Remove foam packing material from the top of the instrument 5 Remove the Startup Kit and set it aside 6 Lift the detector from the foam support on the pallet and carefully set the unit down on a benc...

Страница 33: ...e Rotary Pump 1 Place the rotary pump on the floor within 5 feet of the instrument Place the PTFE drip tray beneath the pump 2 Fill the pump with oil a Remove the filler plug b Pour oil into the pump...

Страница 34: ...oil mist filter assembly to the pump exhaust port Use an NW25 center ring and clamp 8 Connect an NW25 nozzle fitting to the oil mist filter assembly Use an NW25 center ring and clamp 9 Connect the 12...

Страница 35: ...to the pump s inlet port When fitted the tube must be free of kinks or tight bends 6 Cut the tubing ensuring the cut ends are burr free and square 7 Lubricate the restrictor with oil then insert it in...

Страница 36: ...ut a length of 10 mm tubing long enough to connect the instrument to the drying gas exhaust bottle Connect one end of the tubing to the exhaust port on the rear panel Connect the other end to one of t...

Страница 37: ...eedle port fitting onto injector port 6 3 Connect the 10 L injection loop between injector ports 1 and 4 4 Connect the LC system tubing to injector port 2 STOP Attention The instrument requires two se...

Страница 38: ...from the electrospray probe tip 3 Slide the probe into the hole in the probe adjustor plate until the probe body rests on the probe adjustment flange The probe identification contacts must touch the...

Страница 39: ...rument 1 Connect one end of the network cable to the appropriate port on the rear panel of the detector 2 Connect the other end of the network cable to the port labeled ZQ on the workstation rear pane...

Страница 40: ...solution and connect the Rheodyne 9013 needle port fitting to the PEEK union finger tightening it 3 Clip the ground cable with the plug in clip located on the lower right side of the front panel into...

Страница 41: ...CI mode 3 1 Opening MassLynx and Starting the Instrument 1 Double click the MassLynx V4 0 desktop icon to open the application The MassLynx Login dialog box appears Figure 3 1 Figure 3 1 MassLynx Logi...

Страница 42: ...ar 4 The shortcut bar should appear in the MassLynx Main window and Instrument should appear at its top If it fails to appear click Shortcut in the toolbar to open it Then click Instrument at the left...

Страница 43: ...ration and adduct formation Table 3 1 ES Source Page Parameters Parameter Description Capillary voltage Enhances or suppresses ion density by supplying excess charge to droplets Optimal voltages 2 to...

Страница 44: ...s for Operate Source temperature See Table 3 2 Desolvation temperature See Table 3 2 Desolvation gas flow Optimizes gas flow depends on mobile phase composition and flow rate 100 L hr Cone gas flow He...

Страница 45: ...3 4 Figure 3 4 Syringe Selection Dialog Box Parameter Suggested Value Capillary kV 3 5 Cone V 60 Extractor V 3 RF Lens V 0 5 Source Temp oC 80 Desolvation Gas Flow L hr 300 Desolvation Temp oC 150 Con...

Страница 46: ...peak resolution With span settings at 5 the bases of mass peaks as they appear on the Tune window Analyser page should measure 1 da Note The parameters LM Resolution HM Resolution and Ion Energy optim...

Страница 47: ...calibrate Otherwise any data the instrument acquires could fall outside the calibration mass range 3 3 Tuning in APCI Mode After you tune and calibrate see Chapter 4 for calibration information in ESI...

Страница 48: ...the APCI nebulizer and desolvation gas lines at the front panel 7 Open the source enclosure cover 8 Remove the blanking plug from the corona pin mounting contact and fit the corona discharge pin Figur...

Страница 49: ...CI probe to the tee s third port 3 3 3 Specifying Parameters on the APCI Source Page Table 3 4 describes the APCI Source page parameters Table 3 4 APCI Source Page Parameters Parameter Description Cor...

Страница 50: ...lick API Gas in the Tune window to toggle the nitrogen flow to On Note If you do not observe any peak activity the instrument may not be in operational mode To remedy this click Press for Operate STOP...

Страница 51: ...ow rate is 10 L min Turn the probe adjustor see Figure 2 8 in either direction to optimize peak intensities Parameter Suggested Value Corona A 0 1 Cone V 48 Extractor V 3 RF Lens V 0 0 Source Temp oC...

Страница 52: ...the Source and Analyser pages or limit their display to those that are out of range 1 Select Options Readbacks to open the Readbacks dialog box Figure 3 8 Figure 3 8 Readbacks Dialog Box 2 Evaluate r...

Страница 53: ...Figure 3 9 Diagnostics Page Readbacks 3 Let the ion beam stabilize for 3 to 5 minutes 4 Monitor for mass peaks which should appear at approximately the mass values you specified on the ES Source or AP...

Страница 54: ...Before calibrating you must remove the current calibration file and select a reference file 1 Select Calibration Calibrate Instrument from the Tune window Figure 3 3 The Calibration window appears Fi...

Страница 55: ...bes how to specify calibration parameters in these windows and dialog boxes Tune window Instrument Threshold Settings dialog box Automatic Calibration Check dialog box Calibration Parameters dialog bo...

Страница 56: ...e Figure 3 3 5 Click the ES Source tab and enter these suggested parameters in the corresponding fields of the ES Source page Figure 4 3 Row Mass ZQ 2000 Mass ZQ 4000 Span Gain 1 172 9 172 9 5 8 2 772...

Страница 57: ...ick the Analyser tab to open the Analyser page Figure 4 4 and enter these suggested parameters in the corresponding fields Parameter Suggested Value LM resolution 15 HM resolution 15 Ion energy V 0 5...

Страница 58: ...ge These include Capillary Extractor RF Lens and Cone voltages as well as desolvation and cone gas flows 8 Adjust the slide adjustors for LM low mass Resolution HM high mass Resolution and Ion Energy...

Страница 59: ...ialog box appears Figure 4 5 2 Accept the default settings by clicking OK Figure 4 5 Instrument Threshold Settings Dialog Box 4 2 3 Automatic Calibration Check Dialog Box Select Edit AutoCal Check Par...

Страница 60: ...h you should normally avoid setting values greater than 0 20 the default value Apply Span Correction Enabling this option ensures correct mass assignment even when the mass scale differs from the one...

Страница 61: ...e Intensity threshold parameter Polynomial order Once MassLynx matches each peak in the reference spectrum to one in the acquired spectrum it calculates the mass difference the acquired mass less the...

Страница 62: ...og Box Mass measure parameters control conversion of raw continuum data to centroid data which the calibration process requires You must therefore specify them before calibrating in ESI mode Note If y...

Страница 63: ...og box appears Figure 4 9 Figure 4 9 Automatic Calibration Dialog Box 3 Specify the type or types of calibration you intend to perform Static Scanning and or Scan Speed Note For a complete calibration...

Страница 64: ...d the scanning calibration the mass assignment and scan range Specify both Scanning Calibration and Scan Speed Compensation to calibrate for scanning acquisitions over the mass range you specify for t...

Страница 65: ...alibration Acquisitions Setup Parameters Parameter ZQ 2000 ZQ 4000 Scan From amu 50 50 Scan To amu 2040 4080 Run Duration mins 1 0 2 0 Data Type Continuum Continuum Static Span amu 4 4 Static Dwell se...

Страница 66: ...over the selected range when acquiring data for scan speed calibrations see next section for details Inter Scan Delay Specifies how much time elapses between the end of one scan and the beginning of a...

Страница 67: ...ion type for viewing With the required calibration selected MassLynx automatically displays the correct calibration file Figure 4 11 Display Calibration Graphs Dialog Box Note Data for the selected ca...

Страница 68: ...2 Enter changes and click OK Printing the Calibration Report 1 Click the Calibration window Figure 4 1 to activate it 2 Click Yes to save changes in the calibration file The Save As dialog box appear...

Страница 69: ...es listed in Table 4 2 likely apply If the data include these mass peaks but they nevertheless go unrecognized during calibration one or more of the subsequently listed causes probably induced the fai...

Страница 70: ...on screen Calibration report Figure 4 12 for correct peak matches The calibration is correct when the acquired spectrum looks like the reference spectrum and it highlights all the expected peaks Too...

Страница 71: ...ak s highlighting and excludes it from reported data To include a peak as a reference place the cursor over it and right click This matches the selected peak to the closest peak in the reference spect...

Страница 72: ...Calibration 8 Click OK The Automatic Calibration dialog box reappears 9 Click OK to start the verification procedure The instrument performs a scanning acquisition Then MassLynx combines the data acq...

Страница 73: ...olvents change tubing or operate the instrument Refer to Material Safety Data Sheets for the solvents you use and know their chemical properties STOP Attention Do not touch integrated circuit chips or...

Страница 74: ...APCI Weekly ESI Check the rotary pump oil and top off when necessary Weekly Replace the rotary pump oil Every 3 months of continuous operation or whenever the oil appears markedly discolored Clean the...

Страница 75: ...l level appears in the rotary pump s oil level sight glass Check the oil level at weekly intervals Maintain it at or near the MAX level If you must add oil vent and shut down the detector before remov...

Страница 76: ...ed with Oil Mist Filter Note Vent the instrument according to the procedure in Section 5 2 5 and proceed with these steps 1 Turn the instrument s power switch to Off 2 Place something under the pump m...

Страница 77: ...3 3 to start the pump operating Continue to run the pump for 10 to 15 minutes e Select Options Vent and click Yes at the prompt to stop the pump f Let the oil drain completely 7 Use a funnel to add ab...

Страница 78: ...condensate forms in the rotary pump exhaust line When you change the rotary pump oil 1 Shut the vacuum system isolation valve moving its handle fully to the right Figure 5 1 2 When the oil is clear re...

Страница 79: ...nt according to the procedure in Section 5 2 5 and proceed with these steps 1 Turn the instrument s power switch to Off 2 Unscrew the NW25 clamp and remove the exhaust line from the top of the oil mis...

Страница 80: ...8 Reattach the exhaust hose to the NW25 clamp 9 Evacuate the system by selecting Options Pump from the Tune window 5 2 5 Cleaning the Source Assembly Clean the sample cone and cone gas nozzle when the...

Страница 81: ...spare parts when cleaning source components Source Cleaning Procedure 1 Disassemble the source components see Disassembling Source Components on page 82 2 Clean the source components see Cleaning the...

Страница 82: ...STOP Attention Never remove either probe before the source heater cools to below 100 C and the source heaters cool to below 50 C Doing so will shorten the probe heater life To reduce cooling time cont...

Страница 83: ...the message box appears TP02000 1 1 2 4 4 4 4 4 5 3 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 4 4 6 1 Ion Block Fastener 2 BS 10 Black Viton O Ring 3 Screws M3 20 Cross 4 M3 6 PH 5...

Страница 84: ...and retract it from the source Figure 5 7 Figure 5 7 Probe Assembly in Position on the Source STOP Attention If you are cleaning only the sample and cone gas nozzles you need not vent the system Cauti...

Страница 85: ...ont View 9 Unfasten the two clip fasteners Figure 5 7 so that the source enclosure opens downward on its hinge allowing access to the source contents 10 If you are using an APCI probe carefully remove...

Страница 86: ...eedle 11 Remove the PTFE tubing attached to the cone gas nozzle Figure 5 10 Figure 5 10 Ion Block Corona Discharge Needle PTFE Tubing Ion Block Corona Pin Mounting Contact Pumping Block Cone Gas 6 mm...

Страница 87: ...s Nozzle 16 Remove the two 6 mm hex screws that secure the ion block 17 Remove the ion block from the ion block support Figure 5 5 The ion block fits snugly on the support so separating them can requi...

Страница 88: ...aning the Source Components 1 Place the ion block in a beaker with methanol water 1 1 2 Place the beaker containing the ion block and methanol water mixture in an ultrasonic bath for 20 minutes 3 Remo...

Страница 89: ...he pumping block Remove any O rings that remain stuck to the pumping block surface Figure 5 5 2 Use your hand to gently grasp the hexapole Figure 5 13 and retract it carefully from the analyser assemb...

Страница 90: ...5 Replace them if necessary Be sure they are properly installed before you proceed 2 Refit the PEEK seal ring and O ring on the ion block 3 Press the extraction cone into the ion block support Secure...

Страница 91: ...contamination from the probe tip 5 2 7 Cleaning and Replacing the Corona Discharge Needle Clean or replace the needle if it looks corroded or black and you have noticed decreased signal intensity Req...

Страница 92: ...Close the source enclosure cover reattaching its clip fasteners 9 Reinstall the probe assembly 5 3 Replacing Parts 5 3 1 Replacing the Ion Block Cartridge Heater Replace the cartridge heater when it...

Страница 93: ...acing the Heater Cartridge 5 Slide the new heater cartridge into the ion block with the needle nose pliers 6 Place the two heater cartridge ring tags onto the PEEK block terminals 7 Tighten the two te...

Страница 94: ...y fitting 8 Slide a GVF16 ferrule onto the liner tube 9 Press the O ring into the groove facing the short end of the coupling 10 Slide the coupling short end first onto the capillary followed by the n...

Страница 95: ...no nitrogen escapes from the probe tip If you find a leak replace the probe tip assembly and O ring 5 3 3 Replacing the ESI Probe Tip Replace the probe tip if you encounter any of these problems Gas...

Страница 96: ...Butane lighter or match HPLC grade methanol Slotted screwdriver Lint free paper towels Loupe Spare Parts Two GVF004 ferrules part number 700000344 reference number 6070737 Fused silica part number 430...

Страница 97: ...snug in its fitting then gently tug it to be sure it remains in place 16 Tighten the coupling to the probe with a 7 16 inch wrench 17 Run your fingers across the edges of the ceramic capillary cutter...

Страница 98: ...rew that secures the probe tip APCI probe heater spare part part number 700000337 Procedure 1 Loosen the two set screws at the base of the probe tip assembly and slide the probe jacket off the assembl...

Страница 99: ...nded for replacement by the customer Caution Observe good laboratory practice when you handle solvents change tubing or operate the instrument Refer to Material Safety Data Sheets for the solvents you...

Страница 100: ...ry or Waters corporate headquarters in Milford Massachusetts U S A When you contact Waters be ready with this information Completed normal operation checklist for the method you are using Nature of th...

Страница 101: ...2 and Section 3 3 3 Cables are not properly connected Ensure all cables are correctly attached to the source and probe Instrument is not in the operational mode Click Press for Operate Tune window to...

Страница 102: ...in the HPLC system Examine the HPLC system for leaks and correct any you find Broken fused silica capil lary in the APCI probe Replace the fused silica capillary See Section 5 3 4 Source components ha...

Страница 103: ...ge regulator Problem with sample delivery autosampler syringe pump Troubleshoot the autosam pler or check the syringe and syringe pump Source components are dirty Clean source components See Section 5...

Страница 104: ...y low LC back pressure Leaking connector Check all fittings and tighten where necessary Problem with LC solvent delivery Troubleshoot the LC system Broken flow cell in UV detector Replace the flow cel...

Страница 105: ...gh ambient tempera ture or obstructed air flow causes overheating Lower the ambient tempera ture Ensure sufficient air flow around the instrument Remove and clean the fan screens The APCI heater does...

Страница 106: ...Liquid on the baffle plate indicates the temperature is too cold Unstable power supplies in the source supplies or the RF DC generator or unstable photomultiplier supply Check power supplies and RF ge...

Страница 107: ...tainless steel nebulizer tube 8 Connect the LC pump to the probe with a flow of acetonitrile water 1 1 at 1 mL min 9 Make sure the liquid jet flows freely from the end of the capillary and that the LC...

Страница 108: ...an delay times as short as 100 msec The operating software MassLynx version 4 0 lets you select ionization modes and polarities in varying combinations and durations A 1 Preparing for Operation Prepar...

Страница 109: ...move it and replace it with the ESI probe See the Waters ZQ Detector Operator s Guide for details about installing the ESI probe 4 Remove the blanking plug from the mounting contact 5 Install the coro...

Страница 110: ...tion Source 110 A Figure A 2 Installing the Corona Needle A 1 2 Setting Up MassLynx Follow steps 1 through 5 in Section 3 1 to open the MassLynx Tune window Figure A 3 Figure A 3 Tune Window Corona Di...

Страница 111: ...ent output in current APCI mode and current equivalent output in voltage ESI mode Figure A 5 Thus the Current and Voltage values appear to interchange suggesting that current and voltage are simultane...

Страница 112: ...Mode Ionization Source 112 A Figure A 5 Tune Window Diagnostics Page 2 Select Ion Mode ESCi or ESCi to enable the positive or negative ESCi ionization mode Figure A 6 Figure A 6 Selecting the ESCi or...

Страница 113: ...by clicking the drop down list in each row Figure A 7 Note You cannot select polarity from the Ion Mode column Instead click Ion Mode in the menu bar and select mode and polarity from the drop down li...

Страница 114: ...it Appears During ESCi Operation 6 Select MS Method from the Main window shortcut bar Figure 3 2 The MassLynx Function List Editor window appears its list area blank Figure A 9 See the MassLynx 4 0 G...

Страница 115: ...ource 115 A Figure A 9 Function List Editor Window Blank 7 Click The Function n MS Scan dialog box where n the function list number appears Figure A 10 Figure A 10 Function n MS Scan Dialog Box MS Sca...

Страница 116: ...he scan function you just specified Figure A 11 Figure A 11 Function List Editor Window Showing Specified Functions 9 Select Options ESCi mode to disable ESCi mode and resume normal ESI or APCI operat...

Страница 117: ...Daidzein Test Use the Daidzein Figure A 13 test to prepare the instrument for ESCi operation evaluate its performance and determine the ratio of peak heights to the level of noise in a mass chromatogr...

Страница 118: ...lculating the signal to noise ratio as it applies to the ZQ Detector Table A 1 HPLC Conditions Parameter Setting Column 2 1 50 mm Xterra C18 3 m Mobile phase Water acetonitrile 70 30 Flow rate 0 5 mL...

Страница 119: ...RMS APCI Sensitivity Positive ion Typically 1 pg 5 L injection of 0 2 pg L reserpine at 1 mL min flow rate 50 1 S N RMS Dynamic range Five orders of magnitude Spectral stability Mass accuracy to withi...

Страница 120: ...ondensing Shipping and storage temperature 20 to 60 C 4 to 140 F Table B 3 ZQ Detector Dimensions Condition Specification Height 23 in 57 2 cm Depth 26 in 66 1 cm Width 15 2 in 38 1 cm Weight excludin...

Страница 121: ...al Specifications Item Specification Operating temperature range 0 to 45 o C Altitude Up to 2000 m Maximum relative humidity 80 for temperatures up to 31 o C decreasing linearly to 50 at 40 o C Table...

Страница 122: ...fuse 154 002 700001372 Fuse 3 5A SB SMT little fuse 154 03 5T 700001367 Fuse 3A G FA 1 25 x 0 25 in little fuse 312 010 700001362 Fuse 5A SB SMT little fuse 154 005T 700001368 Fuse 8A Anti surge Cera...

Страница 123: ...tem Part Number Adaptor NW25 Hose WAT240495 Clamp Hose 1 in SS Box 10 WAT241163 Clamp Hose 5 8 in SS Box 10 WAT241162 Controller Turbo Pump 24 V 700001127 Edwards 45 Oil 1 Gallon 700000870 Edwards ISO...

Страница 124: ...Butt WAT240828 Table C 4 Valves and Flow Meters Item Part Number Kalrez 0 145 x 0 070 mm 700000903 Kalrez 0 240 x 0 063 mm 70000902 Tailpiece NW25 NW25 700001157 Viton 0 984 x 0 139 700000901 Viton 1...

Страница 125: ...llation Kit 700001290 FractionLynx Dye Kit 716000765 Installation Oil Filters Kit 700001288 Kit Consumable ESI Probe 700000463 PM Kit Protocol ZQ 715000336 Reserpine Kit 700001673 ZQ PM Kit 201000123...

Страница 126: ...k Terminal 700001251 Cone Gas Nozzle ZQ 700001236 Cone Sample ZQ 700001237 Connector PEEK Body Assy 700001202 Cover Plate 700001234 Extraction Cone ZQ 700001238 Fastener ION Block ZQ 700001241 Heater...

Страница 127: ...LCZ ZMD ZQ APCI Knob Grub Screw M3x3 700000579 LCZ ZMD ZQ Attach Screw ESI Probe 700000576 LCZ ZMD ZQ Attach Screw Knob APCI 700000578 M2x2 APCI Grub Screw Slotted 700000967 Screw Flat HD M3x6 WAT0412...

Страница 128: ...RHS ZQ 700001121 Cover Top ZQ 700001119 Guard Fan 120MM RS2584639 700001151 Kit Rebuild Rheo RV700 100 700001303 LCZ ZMD ZQ Glass Eye Loupe 700000883 Molding Front ZQ 700001122 Sample Loop 10 L S S Rh...

Страница 129: ...ration acquisition ranges check 60 Calibration Acquisition Setup dialog box 64 Calibration file setting up 54 Calibration Parameters dialog box 60 Calibration parameters setting 55 Calibration procedu...

Страница 130: ...Display Calibration Graphs dialog box 67 Documentation conventions 24 related 23 Drying gas exhaust bottle installing 36 Duration run 66 E Electrical specifications ESCi 121 Electromagnetic fields 31...

Страница 131: ...nction List Editor window 116 Login dialog box 41 Main window 42 opening 41 security 41 Matched peaks manually editing 71 Maximum standard deviation 60 Miscellaneous components 128 Missed reference pe...

Страница 132: ...ated documentation 23 Relative humidity 31 Removing corona discharge needle 108 hexapole assembly 89 ZQ Detector center panel 108 Replacing APCI probe heater 98 corona discharge needle 91 ESI probe ti...

Страница 133: ...45 Syringe stop 40 T Temperature operating 31 Test Daidzein 117 Threshold intensity 61 Troubleshooting 99 hardware 101 Tune page Analyzer tab 57 settings 55 Tune window 42 110 Analyser page 46 APCI pa...

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