Warner OC-725C, Руководство пользователя

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Publication 5720-001 REV1.0
Unclamping the Cell
Removing the Electrodes
19) To unclamp the cell, turn the GAIN control (CLAMP section) fully counter-
clockwise to the detent off position. This will also disengage the DC GAIN.
NOTE: If the control is not fully off and the DC GAIN is left on, the preparation
will not be unclamped.
20) It is very important that the CLAMP GAIN be returned to the off
position (fully counter-clockwise to click off) as described above
and the CLAMP MODE selector switch be placed in the off
position before removing the current electrode from
the cell.
Failure to perform the above steps will overload the feedback
amplifier (due to the large current generated when the
membrane resistance between the current electrode and the
bath virtual ground goes to zero). This will damage the oocyte!
For this reason we recommend that the user enable the audible
overload alarm to provide a warning when the potential for
such damage exists.
A Procedure for Recording from Oocytes