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6.2   CALIBRATION ADJUSTMENT

 

Depending  upon  use,  we  recommend  checking  of 

calibration every six months. However this can be adjusted to 
individual requirements.

6.3   PROCEDURE TO CHECK CALIBRATION 

The  pipette  is  checked  at  maximum  volume,  at  50  %  of 
maximum  volume  and  at  minimum  or  10%  of  maximum 
volume, whichever is higher.
• 

A new tip is first pre-wetted 3-5 times and a series of ten 

 

pipetting is done at each volume.

• 

Use of forward pipetting technique is recommended.

- 10 -

6.1   DEVISE REQUIREMENTS AND TEST CONDITIONS

An  analytical  balance  must  be  used.  The  balance  selection 
depends upon selected model of the pipette and sensitivity of 
balance reading.
Test  liquid:  Water,  distilled  or  de-ionized,  grade  3  water 
conforming  ISO3696  Calibration  should  be  carried  out  in  a 
draft-free room at a constant (±0.5°C) temperature of water, 
pipette and air between 15°C to 30°C. 
The  relative  humidity  must  be  above  50%  especially  with 
volumes  under  50  µl,  the  air  humidity  should  be  as  high  as 
possible to reduce the effect of evaporation loss.
Special  accessories  for  analytical  balance,  such  as  the 
evaporation  trap  are  recommended  for  the  calibration  of 
volumes under 50 µl.

1.  Calibration adjustment is done with the calibration tool
 

provided along with pipette.

2.  Rotate the volume locking lever to the “lock” position so
 

the volume setting mechanism is locked and able to turn

 

the calibration screw.

5.  Rotate the calibration tool counter clockwise to increase
 

and clockwise to decrease the volume.

6.  After adjustment, check the calibration according to the
 

instruction in point number 6.3

7.  Once within permissible error range, remove the
 

calibration tool from pipette and place volume

 

adjustment knob in its original position.

3.    Remove volume adjustment knob by pulling it upwards.

4.    Place the calibration tool into the calibration grooves.

Содержание VWRA613-6688

Страница 1: ...Precise High Performance PHP Pipette...

Страница 2: ......

Страница 3: ...rocedure to check calibration 10 6 3 1 Conversion of weight readings to volume 11 6 3 2 Calculation for in accuracy systematic error 11 6 3 3 Calculation for imprecision random error 11 7 Maintenance...

Страница 4: ...under the scope of in vitro diagnostics and can be used as a diagnostic medical device in related applications Thereby it ful ls the relevant demands of the directive 98 79 EC of the European Parliame...

Страница 5: ...2 SINGLE CHANNEL VARIABLE VOLUME Volume adjustment knob Tip ejector Volume lock Tip ejector pusher Volume range Tip cone Coupler...

Страница 6: ...3 MULTICHANNEL VARIABLE VOLUME Volume adjustment knob Volume lock Tip ejector pusher Volume range Coupler Tip cone Manifold...

Страница 7: ...VWRA613 6692 100 1000 l 1 100 500 1000 3 1 0 6 0 6 0 2 0 2 VWRA613 6694 2 20 l 0 02 2 10 20 3 1 2 0 9 1 5 0 6 0 3 VWRA613 6690 VWRA613 6693 4 2 FIXED VOLUME PIPETTE RANGE VWRA613 6697 VWRA613 6698 VWR...

Страница 8: ...02 0 02 0 1 10 50 100 20 100 200 30 150 300 1 5 10 2 10 20 5 25 50 3 1 0 8 5 1 0 7 3 1 0 6 8 4 2 7 3 2 3 1 5 1 2 1 0 7 VWRA613 6713 VWRA613 6719 Delivery volume is clearly indicated in the volume disp...

Страница 9: ...osition and keep the tips at a constant depth below the surface of the liquid Pre rinse the pipette tip before aspirating the sample by lling and emptying the pipette tip ve 5 times This is important...

Страница 10: ...the volume lock lever to the unlock position so that the volume can be adjusted to the desired setpoint within the permitted volume range Refer following images After pipetting acids or other corrosi...

Страница 11: ...of speci cation The tip angle is also important the pipette should always be used in a position within 20 angle of vertical See gure below 5 4 OPTIMUM IMMERSION DEPTHS Volume Immersion depth 0 1 l 1...

Страница 12: ...at have high viscosity or a tendency to foam easily This technique is also recommended for dispensing very small volumes 1 Press the operating knob until the second stop 2 Dip the tip under the surfac...

Страница 13: ...e of water pipette and air between 15 C to 30 C The relative humidity must be above 50 especially with volumes under 50 l the air humidity should be as high as possible to reduce the e ect of evaporat...

Страница 14: ...e components 7 2 DISASSEMBLING THE LOWER PART 1 Press tip ejector pusher completely down and hold 2 Pull down the tip ejector and release the tip ejector pusher 11 Calculate the inaccuracy and impreci...

Страница 15: ...tissue Allow them to dry Check the tip cone for foreign articles and remove if any Grease the cleaned parts with the approved lubricant provided with each pipette 7 2 1 DISASSEMBLING THE SINGLE CHANNE...

Страница 16: ...Slightly push the spring and pull up tip cone to remove it from the lower rail image 3 2 Carefully release the piston from the upper rail and move upward to remove it image 4 7 5 3 REMOVING THE CHANNE...

Страница 17: ...0 5 10 l Fix volume 10 l Group 1 Variable volume 0 1 2 5 l Fix volume 2 5 l 7 6 LIST OF SPARE PARTS SINGLE CHANNEL PIPETTE DP 037 DP 0262 DP 0159 SP 0158 DP 022 DP 022 SP 0160 DP 0159 DP 0261 DP 036 D...

Страница 18: ...Group 3 Variable volume 2 20 l Fix volume 20 l Group 4 Variable volume 5 50 l Fix volume 25 l 50 l DP 029 DP 0269 DP 0263 DP 038 SP 0152 DP 023 DP 033 DP 0270 DP 0264 DP 039 DP 0361 DP 0152 DP 0160 DP...

Страница 19: ...ariable volume 10 100 l Fix volume 100 l Group 6 Variable volume 20 200 l Fix volume 200 l DP 035 DP 0270 DP 0363 DP 0154 DP 0162 DP 026 DP 041 DP 0266 DP 034 DP 0270 DP 0362 DP 0153 DP 0161 DP 025 DP...

Страница 20: ...17 Group 7 Variable volume 100 1000 l Fix volume 250 l 500 l 1000 l DP 34 DP 271 DP 042 DP 265 DP 0155 DP 0163 DP 027...

Страница 21: ...ariable volume 0 5 5ml Fix volume 2ml 5ml Group 9 Variable volume 1 10ml Fix volume 10ml DP 020 DP 0268 DP 0272 SP 0168 SP 0060 RP 0156 SP 0110 DP 047 DP 021 DP 0268 DP 0273 SP 0168 SP 0044 SP 0162 SP...

Страница 22: ...M 277 E DPM 364 DPM 364 DPM 365 DPM 366 DPM 367 DPM 368 F SPM 152 SPM 154 SPM 101 SPM 155 SPM 150 SPM 093 G DPM 278 DPM 278 DPM 278 DPM 278 DPM 278 DPM 278 H DPM 128 DPM 129 DPM 130 DPM 131 DPM 132 DP...

Страница 23: ...e is inaccurate Inaccurate dispensing with certain liquids Worn o ring or seal Worn o ring or seal Tip cone is loose Damaged tip cone Improper assembly Tip incorrectly attached Calibration altered Cal...

Страница 24: ...Avantor Performance Materials India Limited 17th Floor Building No 5 Tower C DLF Cyber City Phase III Gurgaon 122002 Haryana...

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