7
Note
:
For hybridization cycles only, you must not use the option of delay Start to avoid that
the probe remains on the tissue without the appropriate temperatures for the hybridization
process, since, if the hybridization process does not start right away, the preparation will remain
at room temperature up to the start time. In this case, you must start the hybridization process
immediately.
For the Pretreatment of the samples in the Semiautomatic or fully Automated FISH, you must
use the delay start to perform the process overnight.
When the process starts, the user must make sure that both
the wash buffer and the waste
capacity are sufficient
. To do this, the system displays a screen with the necessary total volume
for both and once checked, the user must press
OK
to start the process.
6
th
FINISH STAINING PROCESS
The system will mark in light green the slides that completed the staining successfully and will
name them as “
Finished”
. After completing the last step of the process, the systems unlock the
MD-Stainer’s door and will generate a notification with alarm for the user:
"Process
successfully Ended"
.
Once the whole staining process has finished, the modules used will remain closed, keeping all
the preparations hydrated. In order to take the preparations from the instrument, press “Ok” in
the notification
“Process successfully Ended”
and press
Load / Unload Slides
to open the
modules
.
Once the process of
automatic FISH
is finished, the user must remove the preparations and
place them in a cuvette with distilled water and wash them for 30 seconds away from light.
Later, let the preparations dry in the dark and mount them with fluorescent contrast medium,
Fluorescent Contrast Agent - DAPI+AntiFade (Reference: MAD-FS0106-1).
Once the
Semiautomatic FISH
process is finished, the user must remove the preparations and
place them in a cuvette with distilled water and continue with the post-hybridization washing
process recommended, Post-Hybridization Wash Buffer (Ready to use) (Reference: MAD-
FS0105-1). Later, let the preparations dry in the dark and mount them with fluorescent contrast
medium, Fluorescent Contrast Agent - DAPI+AntiFade (Reference: MAD-FS0106-1).
