3
HEATING
-
The preparations must be
heated right before
introducing them into the MD-Stainer at
60ºC
for
at least 1 hour
. Following these guidelines, the sections will adhere correctly and most of
the extra tissue paraffin will be eliminated.
-
The preparations may be kept in the heater overnight in case the sections are prepared the
previous day to the MD-Stainer start-up.
INSTRUCTIONS FOR USE OF THE EQUIPMENT
The MD-Stainer system allows us to work the fluorescence “in situ” hybridization in two ways:
-
Fully automated – It includes the whole process, that is: pretreatment of the sample,
dispensing of the probes, hybridization and post-hybridization washes.
-
Semi-automatic – The first stage is the pretreatment of the sample, followed by the dispensing
of the probe by the user manually and a second stage consisting of the denaturation and
hybridization of the probe in the instrument. Once the cycle is finished, the user must perform
the post-hybridization washes outside the equipment (MAD-FS0105-1 - Post-Hybridization
Wash Buffer (Ready to use)).
Both forms are described individually in each step of the process of the equipment’s use.
Once the MD STAINER and the computer are on, we must access the following icon to run the software
which allows controlling the platform:
Note:
it is always recommended to turn on
the instrument first and the computer afterwards
.
In the main screen, we have available all the functions to program a FISH cycle we describe in detail
below following the daily work order: