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QUICK GUIDE TO TECHNICAL INSTRUCTIONS FOR

USE AND CLEANING OF MD STAINER FOR

FLUORESCENCE IN SITU HYBRIDIZATION (FISH)

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Содержание MD STAINER

Страница 1: ...QUICK GUIDE TO TECHNICAL INSTRUCTIONS FOR USE AND CLEANING OF MD STAINER FOR FLUORESCENCE IN SITU HYBRIDIZATION FISH ...

Страница 2: ...wing guidelines In the picture below the obligatory position for the tissue is marked with a red rectangle The area highlighted in red shows the hybridization area That is why placing the section in the indicated area is important PREPARATION AND PRETREATMENT OF PARAFFIN EMBEDDED SECTIONS Section Paraffin embedded tissue sections are recommended to be 2 3 µm thick maximum and to place them on the ...

Страница 3: ... washes Semi automatic The first stage is the pretreatment of the sample followed by the dispensing of the probe by the user manually and a second stage consisting of the denaturation and hybridization of the probe in the instrument Once the cycle is finished the user must perform the post hybridization washes outside the equipment MAD FS0105 1 Post Hybridization Wash Buffer Ready to use Both form...

Страница 4: ...PREPARATIONS IN THE EQUIPMENT The preparations must be placed in the equipment as the incubation modules are arranged that is horizontally with the label outwards the equipment by pressing them slightly so that they remain stuck in the support points of each module 3rd SCAN SLIDES Its allows the scanning of the preparations previously labelled and placed in the equipment If we choose this option w...

Страница 5: ... do this the user must use the option Assign protocols again to assign the hybridization protocol you wish to use according to the type of probe The standard protocol for the probes supplied by Vitro SA is named FISH HIBRIDACIÓN by default 4th SCAN REAGENTS Once the techniques to perform have been scanned and assigned manually the user must Scan Reagents When clicking on this option a screen will ...

Страница 6: ...ss Both for automatic and semiautomatic FISH the user must proceed with all the scanning process of reagents as described above The only difference between both processes Automatic and Semiautomatic is the requirement of placing the ready to use probe in the reagents rack for the automatic FISH since for the Semiautomatic FISH the probe is dispensed manually by the user being the user who must onl...

Страница 7: ... process the systems unlock the MD Stainer s door and will generate a notification with alarm for the user Process successfully Ended Once the whole staining process has finished the modules used will remain closed keeping all the preparations hydrated In order to take the preparations from the instrument press Ok in the notification Process successfully Ended and press Load Unload Slides to open ...

Страница 8: ...protocols For more detailed information on this section please consult the User Manual There is a function we must mention in this quick guide It is the one which allows the user to adjust the Pepsin time that is the digestion time for each of the probes available in automatic format To access this option you must click on System Editors Protocol Editor FISH and adjust the pepsin time for each pro...

Страница 9: ...ring the cleaning you spot any anomalies on the chamber such as cracks and or degradation when pressing the chamber you must replace it with a new incubation chamber Reference MAD CH36 Incubation Chambers for MD Stainer 3 THOROUGH CLEAN OF THE CHAMBERS If you only use the MD Stainer to perform FISH automatic or semiautomatic there is not a specific periodicity for the thorough cleaning of the cham...

Страница 10: ...responding to Z2 can accumulate waste of some reagents with the prolonged use of the instrument It is recommended to inspect regularly and visually both tubes Z1 and Z2 and perform the cleaning if necessary The system itself also tracks the number of determinations performed before the cleaning cycle By default 400 determinations are programed once this figure is reached The system will then autom...

Страница 11: ...show a 20 minute countdown After 20 minutes the instrument will initialize the system automatically and will purge the waste of the cleaning solutions Click on Cancel anytime to purge the tube waste immediately Preventive maintenance by the Technical Service The preventive maintenance will be carried out for the whole system by a trained technician Contact your MD Stainer supplier to program the m...

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