© Ultrafast Systems LLC /Vernier Software & Technology
24
Figure 9:
Absorption (left) and emission (right) kinetic profile including the
scattered excitation light picked up by the detector
The processed
∆
A
vs
. time data (Figure 10) can now be fitted by the algorithms representing first
order and second order behavior. To do this you will need to write transforms derived from Equations 11
and 12, and test the data against the transformed data. In many spreadsheet programs, including
Logger
Pro
, the requirement for the fitting transform is to express the absorbance as a function of time
with rate constant, initial intensity, and any significant baseline offset as fitting parameters.
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
0.0
0.1
0.2
0.3
A
Time / ms
Figure 10:
Decay portion of the
A
-time profile
-0.05
0.00
0.05
0.10
0.15
0.20
0.25
0.00
0.01
0.02
0.03
0.04
0.05
I/ V
Time / ms
-0.05
0.00
0.05
0.10
0.15
0.20
0.25
0.0
0.1
0.2
0.3
A
Time / ms
