10
Setup
Begin by turning on the
substage illuminator
with the
power switch
located on the
lower left side of the instrument. Set the
objectives
so they are in the approximate
position for actual use. This is best achieved by placing a slide on the
stage
,
rotating the 100X
objective
into position, then raising the stage (via the
coarse/
fine focus control
knobs) until the tip of the 100X
objective
is just above the slide
(almost touching).
Rotate the
nosepiece
until the 10X
objective
is in the light path, then rotate the
phase annulus turret
(the dial on the front of the condenser) in the
phase contrast
condenser assembly
until the “10” is seen in the viewing window (see figure 5).
Remove an
eyepiece
from one of the
eyetubes
and replace with the
phase contrast
centering telescope
(see figure 6).
After loosening the set screw on the
phase contrast centering telescope
, look
through the
phase contrast centering telescope
and
pull out the uppermost piece
of the
phase contrast centering telescope
until the image is in focus. Tighten the
set screw.
The image seen through the
phase contrast centering telescope
should resemble
rings superimposed on one another (see Figure 7A). What is actually being viewed
are the phase rings.
Turn the
condenser centering knobs
, which extend from the condenser mount, until
the two rings of light are centered upon one another (see Figure 7B).
Once the phase rings are centered, remove the
phase contrast centering telescope
and replace with the
eyepiece
.
The phase rings are now centered for the other remaining
objectives
. This process shouldn’t need to be repeated for
each objective setting, although it is advised to perform off and on checks with the
phase contrast centering telescope
to confirm that the phase rings are still centered.
NOTE: Brightfield work can be achieved on models with a phase contrast condenser. The “0” setting on the
phase annulus turret
is used for this purpose.
Aligning the Phase Contrast Annulus Rings
1
2
3
4
5
6
7
Note: This completes the setup for the 1423PH and the 1433PH. The next 3 pages will explain how to use and make
adjustments to the microscope.
1400PH Phase Contrast Models
Phase contrast is a system which involves a series of light baffling annular rings. Proper
alignment of these rings is absolutely necessary to achieve optimum phase contrast.
Figure 7B
Figure 7A
8
Figure 6
Figure 5
