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Section 3 | Data Acquisition

©

2022

– Valeport Ltd

Page | 14

DataLog x2 will now interrogate the instrument and get the settings and display them, then select
Next:

Set your operational mode and Instrument Range setting, then select Next:

Hyperion Fluorometers supplied after November 2020 (Serial No: 75100) do not require the range
to be set. Ignore this setting, if you get an error concerning #400 commands simply ignore it, see
above. Select Next

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Содержание Hyperion Chlorophyll a

Страница 1: ...not be copied reprinted or reproduced in any material form either wholly or in part and the contents of this document and any method or technique available there from must not be disclosed to any other person whatsoever without the prior written consent of the Company Valeport Ltd St Peter s Quay Totnes Devon TQ9 5EW United Kingdom Phone email Web 44 1803 869292 sales valeport co uk www valeport c...

Страница 2: ...ion with 400 Series Instruments 17 5 Electrical 18 5 1 Connector Pin Out 18 5 2 0400 Series Interface Cable 18 6 Communications 19 6 1 Data Output Format PVHYP Pre November 2020 19 6 2 Data Output Format PVHY2 Post November 2020 20 6 3 Operating Modes 21 6 3 1 Mode C Continuous 21 6 3 2 Mode M Continuous Measurement 21 6 4 Hash Codes 22 7 Care and Maintenance 23 8 Software 24 8 1 DataLog x2 24 8 2...

Страница 3: ... also be integrated into Valeport s SWiFT rapidPro CTD and fastCTD profilers and interfaced to MIDAS CTD type instruments The Hyperion has an accurate single channel detector which can be used for many different fluorophores It is designed for integration into systems providing electrical power and delivers a signal that has been correlated to a known concentration of fluorophore 1 1 Fluorophore N...

Страница 4: ...citation 470 nm Detection 696 nm Dynamic Range 0 800 µg l pre SN 75100 2 gain settings 0 40 and 0 800 software controlled Instrument Detection limit 0 025 µg l Actual Detection limit 0 025 µg l Linearity 0 99 R2 Response Time 0 03 to 2 sec 3x SD in RO water calibrated against Chlorophyll a in acetone solution 2 1 2 Fluorescein Uranine Performance Excitation 470 nm Detection 545 nm Dynamic Range 0 ...

Страница 5: ... Phosphate buffer solution 2 1 4 Rhodamine WT Performance Excitation 520 nm Detection 650 nm Dynamic Range 0 1000 ppb pre SN 75100 2 gain settings 0 50 0 1000 software controlled Instrument Detection limit 0 01 ppb Actual Detection limit 0 06 ppb Linearity 0 99 R2 Response Time 0 03 to 2 s 3x SD in RO water Calibrated against Rhodamine WT solution 2 1 5 Sulforhodamine B Performance Excitation 520 ...

Страница 6: ... on fluorophore properties optical filters LED power sample volume and optical path length Prior to November 2020 Serial No 75100 Hyperion Fluorometers had a calibrated linear response for 2 gain settings e g the ranges 0 40 µg l G5 and 0 800 µg l G1 for chlorophyll a Post November 2020 a single gain setting for full scale was introduced At higher concentrations unlike analogue devices which gener...

Страница 7: ...he temperature of the sample increases the fluorescence decreases that is fluorescence is sensitive to temperature In order to improve accuracy measure the sample at different temperatures and derive corrections for changes in temperature Photo bleaching or fading is the permanent degradation of a fluorophore molecule by light resulting in lower signal levels Photo bleaching is dependent on exposu...

Страница 8: ... sensor data is labelled NTU while the Backscatter sensor data is labelled as BTU Backscatter Turbidity Units This is done purely to clarify the difference is sensor technology BTU is not an industry recognised unit but can be considered to be equivalent to the NTU because both sensors are calibrated with the same standard solutions source Please contact Valeport for further clarification if requi...

Страница 9: ...al Characteristics Materials Titanium with sapphire glass window Polymer optical fibre Depth Rating 6000m Dimensions 40mmØ x 179 5mm including connector Weight 0 50 kg in air 0 26 kg in water Operating Temperature 60 C max without damaging the optical sensor 2 5 1 Dimensions As part of a miniTYPE instrument ...

Страница 10: ...directly at the bottom Valeport can provide an Ambient Light Shield 0901SA7 Please contact Valeport for details 3 1 Setting the Gain Prior to November 2020 Serial No 75100 a Hyperion was set to the default range for its specific analyte before leaving Valeport After November 2020 MK2 PCBs were installed in all Hyperions and that removed the need to set the gain If you have bought your Hyperion aft...

Страница 11: ...osts This setting has no effect on Hyperion Fluorometers with MK2 PCBs All Hyperions supplied after November 2020 Serial No 75100 have MK2 PCBs If you get an error concerning 400 commands simply ignore it using DataLog x2 it will look like this 034 0 022 1 1 19 002 1 126 8 128 NTU 040 M1 032 0903705A9 hyperion Nov 2 2020 14 03 038 Valeport tests 401 ERROR PROTECTED 401 ERROR PROTECTED 401 ERROR PR...

Страница 12: ... detection limit Fast data rates should only be used w here good signal levels are encountered otherwise features may be lost in the background noise In very low signal conditions signal noise ratio issues will therefore limit the maximum vertical speed and resolution when running profiles 3 3 Functional Check To perform a functional test connect the Hyperion to both power and PC using the supplie...

Страница 13: ...Hyperion Optical Sensors Operating Manual MANUAL 1013637447 3 issue 1 6 Page 13 2022 Valeport Ltd Leave this dialogue in its default settings then select Next ...

Страница 14: ...tings and display them then select Next Set your operational mode and Instrument Range setting then select Next Hyperion Fluorometers supplied after November 2020 Serial No 75100 do not require the range to be set Ignore this setting if you get an error concerning 400 commands simply ignore it see above Select Next ...

Страница 15: ...rating Manual MANUAL 1013637447 3 issue 1 6 Page 15 2022 Valeport Ltd If there are any new settings to be up loaded to the instrument pressing Finish will send them Check that data is being received field 4 see Data Output Format ...

Страница 16: ...res The magnitude of the data received increases when the supplied fluorescent target is held at 45 to the sensor window By altering the angle of the target the magnitude of the reading should change thereby showing the correct operation of the sensor If the test target is missing then a piece of good quality white paper can be used ...

Страница 17: ... CTD it should be configured using DataLog x2 software as follows Baud Rate 38400 059 38400 Mode Continuous The 400 series Instrument must be configured using DataLog Pro ver 04007125F1 or later Ensure the 400 unit internal Power source from 0400550 for the Hyperion is connected as per cable form 0400C321 Ensure the latest version of Hyperion interface code 04007181 is installed in the Quartzonix ...

Страница 18: ...able 485 6 V Note to enable RS485 comms link pins 1 and 5 5 2 0400 Series Interface Cable END 1 Hyperion SUBCON MCBH6F END 2 0400513 Quartzonix Interface PCB END 3 0400550 PSU Switching PCB FUNCTION PIN CONNECTOR PIN CONNECTOR 1 J3 6 WAY FCI 2 RS232 GND 2 3 RS232 RX in to logger 3 1 RS232 TX out of logger 4 J2 2 way FCI Hyperion Power ve 5 NO CONNECTION Blank off to prevent shorting 6 J2 2 way FCI...

Страница 19: ...ol 8 data bits 1 stop bit No parity No flow control 6 1 Data Output Format PVHYP Pre November 2020 The Hyperion outputs a single NMEA style data string Fluorometers pre SN 75100 Example PVHYP 01 01 1234 45 1 2345 01 C02 7F Where Field Number Description Type Description 1 NMEA Header String Valeport HYPerion 2 Instrument ID Integer 3 Parameter ID Integer 1 Chlorophyll a 2 Fluorescein Uranine 3 Rho...

Страница 20: ...in Uranine 3 Rhodamine Sulforhodamine B 4 Crude Oil 7 Phycocyanin freshwater Blue Green Algae 8 Turbidity 4 1st Parameter Mean Float 5 1st Parameter SD Float 6 1st Parameter Units String ug l for Chlorophyll a NTU for turbidity nephelometer 4 2nd Parameter Mean Float 5 2nd Parameter SD Float 6 2nd Parameter Units String NTU for turbidity backscatter 7 Operating Mode String C02 default 8 Check Sum ...

Страница 21: ...C is the default setting for Hyperion In this mode the instrument will be set into a continuous output cycle of appropriately meaned and filtered data Mode C02 C zero 2 will output a reading every 2 seconds This is the maximum synchronous observation measurement period Mode C04 C zero 4 will output a reading every 4 seconds but that will be a mean of 2 2 second synchronous observation measurement ...

Страница 22: ...nstrument 037 site_info Enter site information 038 site_info Read the site information 039 set_sampling_mode Set sampling mode without running 040 last_sampling_mode Read last sampling mode 059 baud_rate Baud rate 2400 4800 9600 19200 115200 or 230400 082 rs_485_selected Selects if in RS485 mode Usually done with control line 083 rs_485_selected Read status 102 user_output Enter output item 103 us...

Страница 23: ...pt clean Cleaning the optical window should be performed using warm soapy water and optical tissue then rinsed with clean water Organic solvents should not be used to clean the Hyperion Strong acids and bases should be avoided If fouling of the window is considerable the unit should be returned to Valeport for servicing The instrument should be washed in fresh water before storage in the equipment...

Страница 24: ...ated with a button such as Configure for EnviroLog see section 8 2 3 that will set the Hyperion up for operation with an EnviroLog system Configure is available from Valeport Download search for Hyperion from the dropdown list download and install the software is licence free The Configure App will check for the software installed on the device and alter the functionality available as required As ...

Страница 25: ... button 8 2 1 1 Valeport Configure App Without an Instrument If you do not have an instrument or would like to setup a configuration file to load into another instrument in preparation for a future deployment select the tick box on the opening dialogue Offline Mode This will give you access to all the configuration options and allow you to store a configuration profile vps file for later upload to...

Страница 26: ...n and replies from the instrument in the Terminal Window across the bottom of the screen If the terminal window is not visible click on the down arrow to show it You may have to click and drag the window to show all data fields and the terminal window The Terminal window can also be used to send specific commands to the instrument should that be necessary Type into the bottom line in the window an...

Страница 27: ...t Address and the field label and Tab title will be bold to indicate the change Hover over the field you have just updated to see what the current setting is and what is set in the instrument Device Once you have made all your changes press to upload the new configuration into the instrument To set the instrument into a Run Mode select the button at the bottom of the screen 8 2 3 Configure with Op...

Страница 28: ...n with an EnviroLog system simply click on the button You can follow the setup commands in the terminal window Once complete the Hyperion is setup and ready to be interfaced to the EnviroLog system You must ensure that if you are using multiple instruments each has a different Address ...

Страница 29: ...B Sulforhodamine B Fluorometer Titanium housed 6000m rated Supplied with Y lead power and comms DataLog x2 software Manual and transit case 0901001 R Hyperion R Rhodamine WT Fluorometer Titanium housed 6000m rated Supplied with Y lead power and comms DataLog x2 software Manual and transit case 0901001 PC Hyperion PC Phycocyanin Fluorometer Titanium housed 6000m rated Supplied with Y lead power and...

Страница 30: ...d the CE compliance of the product and negate your authority to operate it This product has demonstrated CE compliance under conditions that include the use of shielded cables It is important that you use shielded cables compliant with the product s conformance to protect from potential damage and reduce the possibility of interference to other electronic devices 10 1 KU Declaration of Conformity ...

Страница 31: ...Hyperion Optical Sensors Operating Manual MANUAL 1013637447 3 issue 1 6 Page 31 2022 Valeport Ltd 10 2 EU Declaration of Conformity CE Marking ...

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