TBS-380 Mini-Fluorometer Operating Manual
8
3.2
Instrument Power Up
To turn on the TBS-380, press the
ON/OFF
button. The instrument takes 5 seconds to warm up. After
the warm-up, the TBS-380 is ready for operation.
To adjust the brightness of the LCD, press the
UP
or
DOWN ARROW
buttons.
3.3
Fluorescence Channels
Choose the appropriate channel for your analysis. To do this, press the
A/B
button to toggle between the
2 channels. The activated channel name will be displayed in the lower left corner of the HOME screen.
UV = Ultra Violet excitation
Blue = Blue excitation
See Appendix A for Optical (Wavelength) Specifications.
3.4
Calibration Standard Value
Before performing a calibration, assign a numeric value between 1 and 999 for your standard solution.
(e.g. enter 100 if your calibration standard is 100 ng/mL).
1. Press the
STD VAL
button. Note: If no action is taken within 5 seconds of pressing the
STD VAL
button the instrument will default back to the HOME screen.
2. Use the
UP
or
DOWN ARROW
buttons to adjust the standard value. Holding the button down will
activate faster scrolling.
3. When finished, press the
ENTER
button to accept the value and return to the HOME screen.
3.5
Calibration
1. Press the
CAL
button.
2. Press the
ENTER
button to start the calibration.
3. Insert your blank and press the
ENTER
button. The TBS-380 will average the blank fluorescence for
5 seconds.
NOTE: Be sure to insert the Minicell adaptor in the correct orientation when the Minicell is used (see
1.3.5).
4. Insert the calibration standard and press the
ENTER
button. The TBS-380 will average the standard
fluorescence for 5 seconds.
5. To accept the calibration, press the
ENTER
button when the calibration is complete to accept the
calibration. If
ENTER
is not pressed within 10 seconds, a dialog will appear asking the user if
aborting the calibration is desired. Press the
UP
or
DOWN ARROW
button to abort or accept the
calibration, respectively.
If desired, press the
ESC
button at any time during steps 1-4 to stop the calibration. The TBS-380 will
return to the HOME screen and default back to the previous calibration.
NOTE:
In the event that the Blank concentration is greater than the Standard concentration, the
following error message will appear. We recommend increasing the concentration of the standard by
10X or examine the blank for high fluorescence background.
Standard<Blank
Recalibrate
