TOSOH BIOSCIENCE 07573 Скачать руководство пользователя страница 1 | Manualshive

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HPLC

HIC Column

TSKgel Phenyl-5PW, Ether-5PW

INSTRUCTION

MANUAL

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Содержание 07573

Страница 1: ...HPLC HIC Column TSKgel Phenyl 5PW Ether 5PW INSTRUCTION MANUAL ...

Страница 2: ... purification do not use for any other purpose Confirm compounds are safe Check that obtained compounds and solutions after separation and purification are safe Proper disposal Dispose of in accordance with local laws and regulations Keep this manual for future reference To help protect your property from potential damage and ensure personal safety please read this manual thoroughly before using t...

Страница 3: ...oduction 1 2 Unpacking 1 3 Installation 1 4 Column Storage 3 5 Sample and Eluent Preparation 3 6 Flow Rates 4 7 Temperature 5 8 Guard Column 5 9 Column Efficiency 6 10 Troubleshooting 7 11 Quality Specifications and Warranty 7 ...

Страница 4: ...n Manual contains crucial information on how to care for and use these columns in the proper manner so as to make most effective use of their high performance capabikities 2 Unpacking Check that nothing is the matter with the appearance of package or the column Fig 1 Appearance of the package Then check that the following documents are attached to the column 1 copy Insruction Manual 1 copy Inspect...

Страница 5: ...bubbles into the column will cause degradation of its performance through the occurrence of channeling etc 3 4 Prevention of Pulsatory Flow This type of column is easily affected by pulsatory flow of the solvent Preferably a pump with no fluctuation should be used If a pump with pulsation must be used connect a pulse damper accumulator to the outlet side of the pump in order to compensate for the ...

Страница 6: ... deionized water 5 Sample and Eluent Preparation 5 1 Replacement of Solvents HIC type columns are filled with distilled and deionized water for shipment Replace this solvent with a suitable solvent using one half of the recommended flow rate shown in Table 1 Since frequent solvent replacement accelerates degradation of column efficiency use the same solvent as far as possible 5 2 pH These columns ...

Страница 7: ...w rete results in improved column efficiency and tends to extend column life Standard flow rates for phosphate or other aqueous buffers are recommended between 0 4 0 8 mL min The flow rates and pressure drops for these columns are suggested below ４ Cat No 07573 07656 13063 08804 14018 08641 08642 14013 14014 14015 Types Steel Column phenyl 5PW phenyl 5PW Glass Column phenyl 5PW Glass phenyl 5PW Gl...

Страница 8: ...cy In such case the original column efficiency can be maintained by connecting a guard column before the column and replacing it if efficiency becomes degraded by the adsorption of material to the guard column For mazimum insurance against such problems a guard column should be used as much as possible We prepared the Guard column kit as the guard columns for TSKgel Phenyl 5PW and Ether 5PW Please...

Страница 9: ...ted by the half peak width method shown in Fig 4 and the following equation N 5 54 Ve W1 2 2 Ve Elution volume W1 2 Half width value of peak h Peak height 9 2 Method of Calculating the Asymmetry Factor Fig 5 Method of Calculating the Asymmetry Factor The asymmetry factor of column As is calculated by the 1 10 h method As b a 9 3 Dead Volume If the dead volume of the equipment or the injection volu...

Страница 10: ...replace the old end fitting with a new one being very careful not to loosen any of the packed gel underneath 10 2 Never Eluted or Eluted Late If the elution time is extremely long it might be caused by adsorption on the column packing Most problems can be avoided by washing the column If the column has not recovered after washing it will not recovered anymore In this case you have to exchange the ...

Страница 11: ... 20 100 20 20 100 Column Dimensions mmID cmL Flow Rates mL min Sample Volume μL Cat No 07573 07656 08641 08642 13063 08804 14018 14013 14014 14015 Types Steel Column TSKgel Phenyl 5PW TSKgel Phenyl 5PW TSKgel Ether 5PW TSKgel Ether 5PW Glass Column TSKgel Phenyl 5PW Glass TSKgel Phenyl 5PW Glass TSKgel Phenyl 5PW Glass TSKgel Ether 5PW Glass TSKgel Ether 5PW Glass TSKgel Ether 5PW Glass 7 5 7 5 21...

Страница 12: ... com HPLC database www2 tosoh co jp hlc hlcdb nsf StartE OpenForm TOSOH BIOSCIENCE GmbH Zettachring 6 70567 Stuttgart Germany Phone 49 711 132570 Fax 49 711 1325789 E mail info tbg tosoh com Web site http www tosohbioscience com TOSOH BIOSCIENCE SHANGHAI CO LTD Room 301 Plaza B No 1289 Yi Shan Road Xu Hui District Shanghai 200233 China Phone 86 21 3461 0856 Fax 86 21 3461 0858 E mail info tosoh co...

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