5-5 Organic Solvent Concentration Range
The concentration of the organic solvent can risen up to 50％.
But be careful to avoid salt precipitation when adding salts to aqueous solution
containing organic solvents.
5-6 Denaturing Agents
Denaturing agents such as urea, guanidine-HCl and detergents can be used.
5-7 Sample
The sample should be dissolved in the starting eluent and filtered through a micro-
pore filter (of eg. 0.5 μm pore size) to protect the column from accumulation of
inmpurties.
6. Flow Rates
Factors such as resolution, analytical time, and column life should be carefully
considered in selecting flow rates. A higher flow rate results in a shorter analyhtical
time. Conversely lower flow rete results in improved column efficiency and tends to
extend column life.
Standard flow rates for phosphate or other aqueous buffers are recommended
between 0.4-0.8 mL／min. The flow rates and pressure drops for these columns are
suggested below :
− ４ −
Cat.
No.
07573
07656
13063
08804
14018
08641
08642
14013
14014
14015
Types
〈Steel Column〉
phenyl-5PW
phenyl-5PW
〈Glass Column〉
phenyl-5PW Glass
phenyl-5PW Glass
phenyl-5PW Glass
〈Steel Column〉
Ether-5PW
Ether-5PW
〈Glass Column〉
Ether-5PW Glass
Ether-5PW Glass
Ether-5PW Glass
7.5× 7.5
21.5×15.0
5.0× 5.0
8.0× 7.5
20.0×15.0
7.5× 7.5
21.5×15.0
5.0× 5.0
8.0× 7.5
20.0×15.0
1.2
8.0
1.0
1.2
8.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
0.5−1.0
4.0−6.0
0.5−0.8
0.5−1.0
4.0−6.0
0.5−1.0
4.0−6.0
0.5−0.8
0.5−1.0
4.0−6.0
1.2
8.0
1.0
1.2
8.0
Column
Dimensions
(mmID×cm)
Max.
Flow Rate
(mL／min)
Recomended
Flow Rate
(mL／min)
Max. Pres-
sure Drop
(MPa)
Table 1 Recommended flow rates
