Filter Cube Assembly
Chapter 2: Assembly Instructions
Rev A, February 26, 2016
Page 3
2.3. Step
3
The TLV-U-MF2 Filter Cube holds a standard set of Dichroic Mirror
(up to 25.2 x 36.0 x 1.1 mm), Emission (Ø25 mm), and Excitation Filters
(Ø25 mm), sold separately. Thorlabs’ MDF-GFP Filter Set is shown in Figure 3.
Figure 3 Dichroic Filter Set for Fluorescence Imaging
Thorlabs’ emission and excitation filters can be easily differentiated by the
thickness of the filter mount rings: emission (3.5 mm) and excitation (5.0 mm).
Note that the dichroic mirror has a beamsplitter coating on one side (see Figure
4).
Figure 4 Dichroic Filter Orientation
When held at an angle, the beamsplitter-coated side will appear uniform in
reflection all the way to the edge. The opposite side, when held at the same
angle, will not have a uniform reflection near the edge. There will be a band of
transmission that appears through the substrate. It is important to determine the
beamsplitter-coated side of the dichroic mirror as there is only one orientation
that will work with the filter cube.
Dichroic Mirror
Emission Filter
Excitation Filter
Edge
Transmission
is Visible
Coating
Extends to
the Edge
