ThermoFisher Scientific invitrogen iSort Series, Руководство пользователя

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58 iSort
™
Automated Cell Sorter User Guide
Sample Line Cleaning
Overview Sample Line Cleaning is a four step cleaning sequence that sanitizes and rinses the
sample lines of the system to prevent cross-contamination and carryover between
samples. The procedure is similar to the cleaning procedure used in the Shut Down
procedure (page 88), but the instrument does not power off when the cycle is
completed.
We recommend that you run the sample line cleaning cycle in between samples or
users. The entire procedure takes approximately 20 minutes to complete.
Required solutions •
10% bleach solution
•
Attune ™ Wash Solution (Cat. No. A24974) (detergent solution used in Step 5 of
the protocol, page 59)
•
Deionized water
IMPORTANT!
10% bleach is defined as a 1:10 dilution of 5.25% sodium hypochlorite
in deionized water. This gives a final concentration of 0.5% sodium hypochlorite
equivalent to 5000 ppm of available chlorine. We recommend using laboratory-
grade bleach. Avoid bleach with additives (such as perfumes).
Run Sample Line
Cleaning
1. On the Maintenance screen, press Cleaning Cycles , then select Sample Line
Cleaning .