ThermoFisher Scientific invitrogen iSort Series, Руководство пользователя

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34 iSort
™
Automated Cell Sorter User Guide
System Preparation System Preparation is a set of procedures that prepares the iSort ™ Automated Cell
Sorter for the Analysis or Sort run and ensures optimal instrument performance.
•
System Preparation is initiated automatically after you have entered the
operator name for the Analysis or Sort (page 33) and it takes 10–15 minutes to
complete with minimal user input.
•
If you have not already done so, a dialog box will remind you to pull out the
collection tray and ensure that no collection tubes are present.
•
During System Preparation, the instrument performs the following functions in
the order listed and shows the progress of each step in real time.
-
Check fluid levels
-
Pre-charge system pressure
-
Align stream
-
Debubble
-
Check pressure
-
System check
-
Check flow stability
-
Check drop formation
-
Adjust deflection
-
Determine charge timing
•
When the system preparation is
completed, the Insert Sample
dialog box appears and prompts
you to load your sample to initiate
sample flow and define regions on
the dot plot for Analysis or Sort.
For detailed instructions on how
to load your sample to start your
Analysis or Sort, see “Load
sample”, page 35.