ThermoFisher Scientific invitrogen iSort Series, Руководство пользователя

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iSort
™
Automated Cell Sorter User Guide 33
System preparation
Before starting •
Make sure that the collection tray is pulled out and there are no collection tubes
present.
•
Place an empty 12 × 75-mm tube into the sample tube holder in the down
position.
Choose application 1. On the Home Screen , press Sort or Analyze to select the corresponding
application.
2. On the first screen of the selected application, choose Cells or Particles .
•
Select Cells for cells that express green fluorescent proteins (GFP), are
labeled with green extra cellular labels (FITC or AlexaFluor
™
488 dye), or
have been stained with green dyes (e.g., Sytox ™ Green dye)
•
Select Particles for fluorescent beads (e.g., Attune ™ Performance Tracking
Beads)
3. When prompted, enter your name in the Operator Name text box using the
alpha-numeric keyboard, then press OK .
The name you enter will appear as the File Owner next to the name and size of
the saved FCS file in the File Viewer screen (see “Manage files”, page 46).
4. After you have entered the operator name, the system automatically initiates
the
System Preparation (page 34).