Thermo Scientific Thermo Scientific Vanquish VC-D40, Руководство по эксплуатации

Страница: 82 / 174

6 • Operation
Page 82 Variable Wavelength Detectors (VC-D40, VF-D40)
Operating Manual
6.5 Preparing the Device for Operation
This section gives information on any additional steps that are required
to prepare the device for operation and sample analysis.
Before Operating the Device for the First Time
Prepare the device for the first-time operation, observing the following:
NOTICE
Flush the system flow path thoroughly before operating the device for
the first time:
• When you install devices or components to the system, always flush
them to waste before connecting them in the system flow path. To
flush the Vanquish modules, follow the instructions in the
Vanquish
System Operating Manual .
• When operating the detector with a flow cell that was stored, the
flow cell may be filled with solvent. Use solvents that are miscible
with this solvent, or use an appropriate intermediate solvent. New
flow cells are dry or filled with isopropanol when shipped.
• To remove the isopropanol from the device components:
¨
Verify that the flow cell and the column are not connected in the
flow path.
¨
Purge the pump and then have the pump deliver flow for a short
time.
• Verify that any air bubbles are completely flushed out of the system
flow path.
• Perform a wavelength validation. If the validation fails, perform a
wavelength calibration.
Before Starting Sample Analysis
Before starting an analysis:
• Check the liquid level in the solvent reservoirs. Verify that the
amount of solvent is sufficient for the analysis.
• Verify that the doors of all modules in the Vanquish system are
closed.
• Observe the guidelines for use of flow cells in Guidelines for Use of
Flow Cells ( } page 84) .
• Make sure that the chromatography system is properly equilibrated
(see further down).