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Using the Spectrophotometer 

8 

Evolution Pro Spectrophotometer User Guide 

Thermo Scientific 

 

 

Tip 

The power light flashes slowly when the spectrophotometer is starting up. When the 

start-up sequence is complete the light will be steady. If the power light starts to flash 

rapidly this indicates an error condition. Check INSIGHT software for any error reports. 

If INSIGHT it not running or shows no error 

1.

 

Power the spectrophotometer OFF. 

2.

 

Perform basic checks and prepare to collect fault information: 
a.

 

Check that both sample and reference beams are clear of obstacles. 

b.

 

Close the sample compartment doors. 

c.

 

Start INSIGHT software on the attached computer. 

d.

 

Power the spectrophotometer ON. 

e.

 

Watch the progress of the startup routine. 

3.

 

If the error repeats, note the stage (bottom left corner of the window) where the error 

occurred and contact us. 

 

 

•

 

Trigger port – connect a contact closure trigger device here 
–

 

Used with the rapid mix kinetics accessory 

–

 

Used with third party remote triggering devices 

•

 

Accessory Port – provides support for planned future accessories 

•

 

USB-B Port – connect the computer running the instrument control software here 

 

Using the Spectrophotometer 

Connecting and Powering the Spectrophotometer 

Refer to the Getting Started guide supplied with the instrument (printed copy) and also on 

the same media as this user guide. 

 

 

Making a Measurement 

Refer to the INSIGHT software help system, included with your installation of INSIGHT 

software. INSIGHT software help includes quick-start guides for each application mode. 

These are available with one click from the help system home screen. 

The beam direction in the sample compartment is from right to left. 

The beam direction in the reference compartment is from front to back. 

Содержание Evolution Pro

Страница 1: ...UV Visible Evolution Pro Spectrophotometer User Guide 269 350000 Revision A August 2021 ...

Страница 2: ...of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be...

Страница 3: ...us situation which if not avoided could result in minor or moderate injury NOTICE Follow instructions with this label to avoid damaging the system hardware or losing data Note Contains helpful supplementary information Evolution Pro Spectrophotometer Considerations Site Preparation and Safety Before using the system read the site preparation and safety manual on the documentation media provided Al...

Страница 4: ... accompagnamento del sistema No opere este sistema sin sequir las precauciones de seguridad descritas en este manual y en la documentación proporcionada con el sistema Operating Precautions The spectrophotometer contains precise optical components Handle it carefully and follow these precautions Do not allow moisture to leak into the instrument interior Wipe off spilled chemicals immediately Do no...

Страница 5: ... hold down screw is generally only required when tubing or hoses are being brought into the sample compartment under the flaps at the bottom of the front cover Installing and Removing Accessories Standard Cell Holder The standard cell holder included with the instrument accommodates 10 mm pathlength cells Accessories that are not electrically connected are called manual accessories It is not neces...

Страница 6: ...elease the plate from the toggle 2 Lift the plate off the alignment pins and remove the accessory from the sample compartment Smart Accessories Smart Accessories include cell changers and sample holders that feature Auto recognition Smart alignment Serial number reporting A connector under the accessory baseplate provides data communication and power Smart accessories are hot swappable It is not n...

Страница 7: ... in a single batch of samples Installing a Smart Accessory 1 Open the sample compartment and remove any cell holder or accessory 2 Insert the accessory a Grasp the accessory by the handles and lower it into the sample compartment aligning the connector under the baseplate with the connector in the sample compartment floor b Line up the two holes at the back of the accessory with pins A and B in th...

Страница 8: ...front cover of the sample compartment has two openings to allow passage of tubing or cables into the sample compartment These openings are covered by a layer of black foam to exclude light Slits cut into the foam allow you to create an opening without allowing excess light into the sample compartment Slits on bottom of panel CAUTION Avoid pinch hazard Keep hands and objects clear of the accessory ...

Страница 9: ...hotographs below For the TPS 1500W or any similar thermostatted recirculator using insulated tubing replace the insulation around the tubing inside the sample compartment The hold down screw at the center of the sample compartment front cover should be secured to hold the cover firmly in place when tubing or cables are present Reference Cell Holder For measurements with a reference sample install ...

Страница 10: ...stage bottom left corner of the window where the error occurred and contact us Trigger port connect a contact closure trigger device here Used with the rapid mix kinetics accessory Used with third party remote triggering devices Accessory Port provides support for planned future accessories USB B Port connect the computer running the instrument control software here Using the Spectrophotometer Con...

Страница 11: ...Always insert cuvettes so that the beam passes through the polished walls Plastic cuvettes generally have a V or other mark at the top of one polished wall Ideally you should always orient this V in a consistent direction relative to the beam Generally this is towards the beam Using the Reference Beam For simple measurements it is not generally necessary to put a reference cuvette containing blank...

Страница 12: ...components that may be damaged by a corrosive environment Quartz windows on the monochromator cover protect the monochromator from corrosive gases in the sample compartment to some extent However the monochromator is not sealed and corrosive gases from the sample compartment can still make their way into the monochromator through other routes There are no windows between the detector compartment a...

Страница 13: ...ternal thermostatted recirculator The heated or cooled liquid flows through internal chambers within the metal block where the cells are held bringing that block to the same temperature as the liquid The cuvettes then come to the same temperature as the block To install a thermostatted accessory 1 Install the accessory in the sample compartment 2 Route the tubing from the recirculator through the ...

Страница 14: ...re replacement within ten years INSIGHT software tracks lamp use and reports remaining lamp life on the Lamp tab in the System Settings Re alignment of the instrument optics is required following replacement of the lamp This means that the process must be completed by a trained and equipped service engineer either in the field or at one of our service depots Live Readings In spectrophotometers wit...

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