Thermo Scientific Dionex Easion, Руководство оператора, Страница: 51 / 126

3 • Operation and Maintenance
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6. Leave the syringe in the port (this prevents the sample from siphoning
out of the loop before injection).
7. Use one of the following methods to inject the sample onto the
column.
•
Manually: Click Inject on the Chromeleon ePanel. For more
information about manual sample processing, see Section 3.11.2 .
•
Automatically: Include the Inject command in a Chromeleon
instrument method (see
Section 3.11.6 ). For more information
about automatic sample processing, see Section 3.11.3 .
Loading Samples with a Vacuum Syringe (Pull Method)
1. Verify that the sample loading port on the front of the Dionex Easion
is connected to sample port
S (5)
on the injection valve (see
Figure 3-6 ).
2. Disconnect the waste line from port
W (6)
on the injection valve and
attach a shorter line: 25 to 30 cm (10 to 12 in) of PEEK or PTFE
(polytetrafluoroethylene) tubing (see
Figure 3-6 ).
3. Place the free end of the line into the sample.
Figure 3-6. Loading Sample with a Vacuum Syringe (Pull Method)
(1)
L
L
S
W
P
C
(2)
(3)
(4) (5)
(6)
Sample
Injection Valve
Sample
Loading Port
(on front door)
Syringe