How to use the VeritiPro
™
Thermal Cycler
Operate the instrument using the touchscreen. For detailed
instructions on using the thermal cycler, see the
VeritiPro
™
Thermal
Cycler User Guide
(Pub. No. MAN0019157).
In the Home screen for the Touchscreen, you can set up a run by
creating a new method for a run. To set up a run using a new
method:
1. Press
New Method
or where it says
Set Up Run
.
2. Select a template.
3. In the edit mode, edit the parameters of the method template
such as temperature, time, number of steps/stages.
4. To access advanced editing options, press
Manage Steps
>
Advanced Options
. Advanced editing options include VeriFlex
™
blocks for optimization, simulation modes, ramp rates, and
AutoDelta.
5. Save the new method in a folder.
6. (Optional) Press
Start Run
to start the Method.
Maintenance guidelines
CAUTION!
During instrument operation, the temperature of
the heated cover can be as high as 110°C, and the
temperature of the sample block(s) can be as high as 100°C.
Before performing the procedure, keep hands away until the
heated cover and sample block(s) reach room temperature.
To ensure proper operation:
• Regularly:
– Wipe the instrument surfaces with a lint-free cloth.
– Clean the vents, touchscreen, and sample block of the
instrument.
– Clean the sample wells with 100% isopropanol.
• Use only consumables recommended by Thermo Fisher
Scientific for the instrument.
Use of consumables that are larger or smaller than the specified
volume can damage the instrument, contaminate the sample
block, and/or decrease the PCR yield (due to inefficient thermal
transfer).
•
Do not use sharp objects on the touchscreen.
Use only your fingers or blunt objects to enter commands on the
instrument touchscreen. Sharp and/or pointed objects such as
writing utensils can damage the surface of the touchscreen.
•
Back up frequently.
Routinely back up the configurations and files on your
instrument to a USB drive. Regular backups protect against
data loss caused by user error, power failure, or instrument
error. For more information, see the
VeritiPro
™
Thermal Cycler
User Guide
(Pub. No. MAN0019157).
How to use the MicroAmp
™
96-well Tray and
Retainer
IMPORTANT!
Use the MicroAmp
™
96-well Tray and Retainer to
prevent crushing of tubes in the thermal cycler.
When small number of tubes (1–2 tube strips or ≤8–16 tubes are
placed in the sample block without support, they can be crushed or
deformed when the lid of the thermal cycler is closed over the
block. Using the tray and retainer set allows the pressure to be
distributed evenly over the tubes and prevents uneven pressure
across the block.
Using the tray and retainer set is optional when using ≥3 tube strips
or ≥16 tubes that are distributed evenly across the block.
• The
blue
tray and retainer set is compatible for use with
MicroAmp
™
single tubes or tube strips
with
separate
cap
strips.
• The
blue
tray by itself is compatible for use with MicroAmp
™
tube strips
with
attached
caps.
• The
black
retainer is compatible for use with
single
MicroAmp
™
reaction
tubes with attached caps
.
Prepare samples using MicroAmp
™
tubes/tube strips with
separate cap strips
The following procedure describes how to properly load and seal
MicroAmp tube strips using cap strips and the MicroAmp 96-well
tray/retainer set. For a visual demonstration, watch the video "How
to use adapter tray retainers" by visiting
.
1.
Separate the
blue
tray and retainer by squeezing the release
catch as indicated in the graphic.
1
2
3
1
1
Release catch
2
MicroAmp
™
96-Well Retainer
3
MicroAmp
™
96-Well Tray
2.
Place the
blue
tray on the 96-well base.
3.
Load the tube strips on the tray.
4.
Pipette the reaction mixture into the tubes.
5.
Place the
blue
retainer over the tubes and snap the retainer
into the tray.
6.
Seal the tube strip using a MicroAmp
™
tube strips with cap strips” on page 4 for instructions.
7.
Remove the
blue
tray/retainer assembly containing the sealed
tube strips from the 96-well base and place the assembly into
the instrument.
1
2
4
3
5
1
MicroAmp
™
8-Cap strip
2
MicroAmp
™
96-Well Retainer
3
MicroAmp
™
8-Tube Strip (0.2-mL) or MicroAmp
™
Reaction Tube
without Cap (0.2-mL)
4
MicroAmp
™
96-Well Tray
5
MicroAmp
™
Splash Free 96-Well Base
VeritiPro
™
Thermal Cycler Quick Reference
3
