5
Maintenance
Cleaning the Check Valves
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
103
Cleaning the Check Valves
If you use buffers with high concentrations of salts, clean the check valves as part of a regularly
scheduled, preventive maintenance procedure every 6 to 12 months depending on your usage.
Clean the check valves if they are not functioning properly or if you experience residual
pulsation that is too high.
To clean the check valves
1. Remove the LDA from the pump body (see
“Removing the LDA from the Pump”
2. Disassemble the LDA (see
).
3. Using a 10 mL syringe with a very short piece of silicon tubing at the Luer exit, gently
flush the check valves with 10 mL of distilled water.
4. Repeat
with 10 mL of isopropanol.
5. Verify that fluid flows freely through the check valve. If you cannot achieve free flow
through the check valve, replace it with a new one.
6. Reassemble the LDA (see
7. Reattach the LDA to the pump (see
“Reattaching the LDA to the Pump”
).
Cleaning the Pistons
Clean the pistons as part of a regularly scheduled preventive maintenance procedure or if you
observe precipitate on them.
To clean the pistons
1. Remove the LDA from the pump (see
“Removing the LDA from the Pump”
2. Disassemble the LDA (see
).
3. Moisten a soft, lint-free cloth with isopropanol and wipe the pistons with it.
4. Reassemble the LDA (see
5. Reattach the LDA to the pump (see
“Reattaching the LDA to the Pump”
).
CAUTION
Do not use an ultrasonic bath to clean the check valves. Sonicating the check
valves can damage their small ruby balls.
Note
If the pistons are scratched or appear cloudy, replace them.