Thermo Electron C3i, Руководство пользователя

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5.4. Operational Limitations
The C3i centrifuge and its associated rotors offer a high level of RCF performance. In some cases the
maximum possible RCF will exceed the one sustainable by the sample container.
The rotor RCF performance displayed is the maximum available in the rotor pockets. Depending on the
sample containers or on other application needs, the maximum RCF which must be used is to be
determined by the operator.
The centrifuge is not explosion proof.
Using this centrifuge with explosive samples is entirely at the user’s own risk. Do not under any
circumstances use the centrifuge in an explosive environment.
5.5. Aerosol Risks
Due to the action of the turbulence created in the bowl, a centrifuge is an aerosol generator.
A ventilated centrifuge (like the C3i) exhausts, into the room, air which has passed through its bowl. Thus
the risk of the spread of aerosols is significant in a ventilated centrifuge.
In the case of samples presenting a biological or chemical risk, the operator must take suitable precautions
to prevent or reduce this risk.
Thermo recommends the use of its sealed buckets, which are certified for bio-containment through
worldwide recognized testing procedures performed in recognized external laboratories.
Aerosols are produced naturally when using uncapped tubes or bottles. They are also produced when a
container deforms or breaks provoking a small or large sample leak.
We draw your attention to the use of tubes in angle rotors. Even if the liquid level does not touch the cap
when in a static state, it could do so during centrifugation and leak. You should therefore follow the
instructions of the tube supplier concerning the fill level and sealing of the tube.
Once a biological risk is known or suspected, samples should be placed in a sealed container. Should such
containers not be available, sealed accessories should be used, such as angle rotors with sealed lids.
C3i-CR3i Hazards, Precautions and Limitations of Use
20
Duration of aerosol presence
When aerosols are created in a centrifuge bowl, they persist in the air for a period of 3-5 minutes after the
rotor stops moving. Indeed, the action of opening the lid itself causes the dispersion of aerosols into the
environment.
When an elevated risk of aerosols or of breakage is perceived the accessories should be handled
using gloves and opened in an environment ensuring the protection of the operator (safety cabinet,
glove chamber, wearing a mask ...), even if they are apparently sealed.