General rules
Keep windows and doors in the working areas closed while work is in progress.
Do not consume drinks, food or smoke in the working areas. Do not keep foodstuffs in the working areas.
Lab coats or other protective garments must be worn in the working area.
Oral pipetting is prohibited. Use pipetting aids.
Only use needles and syringes if absolutely necessary.
With all manipulators, be sure to avoid aerosol formation as far as possible.
On completion of work and before leaving the working area, clean hands thoroughly. Disinfect and remois-
turize hands if necessary.
Keep working areas clean and tidy. Only equipment and materials actually required should be on the work-
tops. Keep stocks in the areas or cabinets provided for them only.
Regularly check the identity of the biological agents used if this is a requirement for hazard potential
assessment. The length of the interval between checks should be set according to hazard potential.
With regard to the handling of biological agents, employees must be issued with verbal instructions relating
to the workplace before commencing work. These instructions must be re-issued at least once annually
thereafter.
Employees inexperienced in the fields of microbiology, virology or cellular biology must be given thorough
instructions and be supervised.
Vermin and pests must be combatted regularly if necessary.
Handling of pathogenes
Also heed the following basic rules regarding the handling of pathogenes:
Disinfect all workplaces on a daily basis. It may be necessary to switch disinfectants as a precaution
against resistant germs.
Do not wear protective garments outside the working areas.
Contaminated equipment must be autoclaved or disinfected prior to cleaning.
Waste containing pathogenes must be collected safely and rendered harmless through autoclaving or
disinfection.
If infectious substances are spilt, the contaminated area must be closed off immediately and disinfected.
If work is conducted using human pathogenes against which an effective antidote is available, all
employees who are not yet immune must be vaccinated and checked regularly for immunity in the appro-
priate manner.
The health of employees must be monitored by medical check-ups, i.e. employees must undergo an initial
check-up before commencing work and subsequent annual check-ups. Medical check-ups should be
conducted in accordance with the applicable employers´ liability insurance association guidelines, in
particular G 24, "Skin diseases", and G 42, "Infectious diseases". As generally recognized rules of indus-
trial medicine, they enable the doctor to assess, evaluate and record the results of examinations according
to the same criteria.
* Apply to cell cultures as appropriate
Source: specification B003, edition 1/92 - ZH 1/343 of the Berufsgenossenshaft der chemischen Industrie (employer´s liability insurance as-
sociation for the chemicals industry), Jedermann Verlag, Dr. Otto Pfeffer OHG, PO box 103140, 69021 Heidelberg
BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEERING PRACTICE *
50 079 041
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Содержание BBD 6220
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