Terragene BIONOVA MiniBio, Руководство пользователя

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Bionova® MiniBio Page 8
POSITIVE CANCELED NEGATIVE
The Bionova® MiniBio allows verifying the remaining in-
cubation time in every reading position by ticket printing.
Whenever a reading process is in course, press and hold
the button for 3 seconds, a ticket will then be printed
informing reading position, incubation program and re-
maining incubation time.
To verify remaining incubation time of 2 or more readings
in course press 2 buttons simultaneously ; the device
renders a ticket informing remaining incubation time of all
positions.
Remaining incubation time
Instructions for use
The positive control should belong to the same
batch of the processed BI.
Use a non-sterilized biological indicator as a po-
sitive control every time a processed indicator is
incubated.
Incubate the processed biological indicator and the
indicator used as positive control as long as speci-
fied in the instructions.
Press the cap to seal the tube and crush the ampoule, make
sure the media completely wets the carrier. Write a C (for
control) and the date on the label. The positive control can
be placed in any position of the Bionova® MiniBio incuba-
tor. The positive control should belong to the same batch
of the processed BI.
It is a good practice to use a positive control, this helps
ensuring:
Rapid Readout
The indicator used as positive control should show a posi-
tive result (red light ). The processed BI results are
not valid until the biological indicator used as positive
control shows a positive result . A processed BI
with a positive result, indicates a failure in the sterilization
process. A negative result, (green light ) after 20
min. (60 ºC), 30 min. (60 ºC), 1 h. (60 ºC), 2 hs. (60 °C), 3 hs.
(60 ºC) 4 hs. (37 ºC) according to the used program, indica-
tes that the sterilization process was effective.
Take action on any positive result immediately. Always
retest the sterilizer and do not use the sterilizer for pro-
cessing loads until 3 consecutive BI results are negative.
Visual Readout
The Rapid readout system allows the confirmation of re-
sults by color change after 48 hours or 7 days incubation
analysis (if 7-day readout is made, an humidified incuba-
tor will be required to avoid media dry out). If the sterili-
zation process was not successful, the growth media con-
tained in the indicator will turn to yellow, as it is shown
in Instruction of Use of BIs. If the sterilization process was
successful, the media will remain unchanged.
The color of the growth media in the BI used as
positive control must turn to yellow during the
process of incubation for the results to be valid.
Record the positive ones and discard them im-
mediately as shown in the biological indicators
instructions for use.
Dispose Bls after use, according to your healthcare and
safety regulations. Positives BIs can be sterilized (see BIs
instructions for use).
Positive Control
Disposal
Interpretation of results
-Correct temperature is reached.
-Viability of spores has not been altered due to improper
storage temperature, humidity, or proximity to chemicals.
-Capability of the media to promote rapid growth and fluo-
rescence generation.
-Proper functioning of Bionova® MiniBio incubator.