Teledyne CombiFlash EZ Prep, Руководство пользователя

Страница: 59 / 245

Section 2 Preparation: Rf + , Rf + Lumen, Rf+ PurIon, and EZ Prep Systems
2-23
a peak after an extension, the system will add another, up to a
maximum of three extensions.
Occasionally, compounds might come off the column once the
%B returns to the minimum value at the end of a run. If the
Run Length Extension is enabled, it will automatically extend
the run one time to clear the column and plumbing of any
remaining material.
Figure 2-10 Automatic Run Extension Examples
Figure 2-10 illustrates the possible run extensions. The
original method was programmed to rise to the maximum %B
over seven minutes, hold for two minutes and return to the
minimum %B for a final minute. At nine minutes, a peak was
eluting. The system extended the run, holding the %B at the
maximum level for another five minutes. This also occurred at
fourteen and nineteen minutes, resulting in the second and
third extensions. At 24 minutes, the %B solvent strength
returned to zero. Before the final minute elapsed, more
compound was detected, causing the system to extend the run
for a final five minutes at the minimum %B.
Enable rapid equilibration – select this option to
equilibrate the column at a high flow rate.
Note
Due to the high pressure that is possible during rapid equilibration,
this option may not be desirable for some column media or
purification methods.
Enable Automatic Peak Hold - inserts an isocratic hold for
the duration on an eluted peak.
0 5 10 15 20 25
Original method
Extension 1
Extension 2
Extension 3
Final Extension
Minutes