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fMRI User Guide

Electrophysiology recording during functional MRI

Updated 2021-01-28

Содержание fMRI

Страница 1: ...fMRI User Guide Electrophysiology recording during functional MRI Updated 2021 01 28...

Страница 2: ...5 Notices The information contained in this document is provided as is and is subject to being changed without notice TDT shall not be liable for errors or damages in connection with the furnishing us...

Страница 3: ...4 Electrophyisology Recording in Functional MRI fMRI Environments using TDT 6 Getting Started 16 MRI Recording Processor Gizmo 21 Physical Setup and Testing 27 Troubleshooting FAQ 29 Post Processing...

Страница 4: ...y from the brain of a subject The data is filtered for both fast neural spiking activity and longer term activity of local field potentials LFP The subject can be head fixed or freely moving In fMRI t...

Страница 5: ...neural responses to stimulus and elucidate the underlying neural activity driving the BOLD response fMRI User Guide 5...

Страница 6: ...See Minimizing Imaging Artifacts for an example of the image distortion TDT non ferrous headstages are available in 16 channel LP16CH ZNF and 32 channel LP32CH ZNF form factors with standard 2m cable...

Страница 7: ...ters cause temporal shifts that make it more difficult for artifact removal Low pass filters cause ringing after the scan which increases the amount of signal lost immediately after the scan MRI Kit T...

Страница 8: ...e RF filter The kits also include 50k resistor test blocks for your headstage type 16 channel or 32 channel Omnetics MRI test resistor blocks Omnetics Omnetics connectors pin savers gender change or a...

Страница 9: ...lkthroughs of the Synapse software These are very helpful for beginner users first learning the Synapse environment Lightning Videos Short unnarrated videos that demonstrate specific actions in TDT so...

Страница 10: ...on RZ and PC Next turn the RZ on The display screen on the processor should illuminate with information about the unit s DSP cards To check whether there is communication between the RZ and the PC op...

Страница 11: ...connected launch Synapse If this is your first time launching Synapse the Rig Editor will appear and it will be blank Click Detect for Synapse to recognize your RZ and the number of cards installed in...

Страница 12: ...GURATION In Synapse in the PZ5 or PZA Subject Interface options set the subamplifier to DC Coupled mode Using DC coupling increases the probability that the artifact removal will be successful and avo...

Страница 13: ...s the subamplifier subject ground to the banana jack on the back of the PZ5 PZA Subject Interface Note that the subject ground should never be connected to the MRI ground See System Grounding section...

Страница 14: ...lifier wifi The amplifiers have built in wifi to allow network updates This must be disabled during the scan and can remain permanently disabled use the Ethernet port to do network updates PZ5 Press t...

Страница 15: ...PZA Subject Interface Press the brightness icon on the touchscreen this turns off the touchscreen display after 5 seconds fMRI User Guide 15...

Страница 16: ...e raw data for offline analysis and artifact subtraction Has special filters that reduce ringing after the artifact blanking period has passed tuned specifically for single unit and local field potent...

Страница 17: ...of the signal to view when an artifact is triggered Make sure this window is long enough to capture the entire artifact you want to remove Optionally save the Timing Epoc to get timestamps when artifa...

Страница 18: ...I gizmo for processing and filtering The outputs for Single Unit and LFP filter signals are saved in Stream Store Processors at the appropriate sampling rates If the MRI has a digital Sync line to mar...

Страница 19: ...e Unit and LFP plots by click dragging the vertical red bars Single Unit and LFP data are handled differently due to the different frequency characteristics of these signals to minimize signal ringing...

Страница 20: ...tion to see the full scanner artifact and signal This can help in setting up the appropriate rejection window and validating the artifact removal Check Bypass on the upper right corner of the run time...

Страница 21: ...d by the PZ5 PZA Subject Interface amp They do not resolve RF noise that is generated along the cable from the PZ5 PZA Subject Interface amp to the MRI Any additional RF noise can be filtered with the...

Страница 22: ...e waveguide there is also often a filter plate for BNC and DB25 connectors on the penetration panel to pass signals between the MRI room and the control room Use the DB25 female and DB25 male connecto...

Страница 23: ...nterface amplifier See Touchscreen Configuration Test the system using a phantom with no RF filter This is your baseline signal If there is significant artifact connect the RF filter to the back of th...

Страница 24: ...GND should be on the MRI or in the gradient supply in the nearby component room Vertical Scanners Some NHP groups have vertical Brukner 4 7 and 9 4 T scanners These scanners present a significant prob...

Страница 25: ...fore and during the scan The scanner artifact can be 100mV on the recording signal MRI artifact is not correlated with the field size The bore diameter will have a bigger effect To do this Perform tes...

Страница 26: ...f there are any image artifacts Repeat the above tests in steps 2 4 with your electrode using an agar gel or saline brain phantom for a complete system test from electrode to amplifier 3 4 With electr...

Страница 27: ...Units artifact rejection and disable the built in filters Add a Neural Signal Referencer gizmo to further clean up signal after the MRI Processing gizmo then filter and store with Neural Stream Proces...

Страница 28: ...the TDT RF filter If the distance from the scanner room to the control room is 5 meters e g vertical bore magnets such as the Brukner 9 4 T it may be necessary to leave the PZ5 PZA in the MRI room In...

Страница 29: ...ee the Lightning Video to see this importing sequence You can also copy the block file path via Windows Explorer https www tdt com lightning Import2Matlab Link to the MATLAB SDK https www tdt com supp...

Страница 30: ...e use wisely Link to Matlab Workbook examples Link to Python Workbook examples If you have other scripting needs please reach out to TDT Tech Support at support tdt com View Data in OpenScope For a fi...