TATA Motors CHECK ExpressPCR, Руководство пользователя

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RUN SETTINGS
The ExpressPCR run settings tab is where you
will determine the optical settings, view your dye
calibrations and access the advanced settings.
OPTICS SETTINGS
The ExpressPCR will acquire optical data during the
hold times. The longer the hold times are the better
your optical data will be. The optical settings can be
separated in these two different categories:
INTERCALATING DYES
These dyes, for example SYBR Green I, Resolight, Eva Green,
are bright reagents and require a shorter integration time.
SEQUENCE-SPECIFIC PROBES
These dyes, for example FAM, VIC and HEX, are commonly
used for TaqMan genotyping or multiplex experiments.
Hydrolysis probes are not as bright as Intercalating dyes
and therefore require a longer integration time.
INTEGRATION TIME
Integration Time controls the exposure time, in seconds,
of the optical sensor inside the ExpressPCR instrument
during thermal cycling. Brighter reagents require shorter
integration times than dimmer reagents.