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Operating Instructions – TN50

1. INTENDED USE

The floor standing open top cryostat TN50 is intended for rapid freezing and cutting

thin sections of frozen specimens for professional use in routine and research

laboratories in the fields of biology, medicine and industry.

The system of knife advance operates very reliable from 0.5 to 100 µm with trimming

function up to 750 µm. The quality of cutting of this microtome is increased by the
automatic retraction during the upstroke of the specimen, which avoids rubbing on the

disposable blades or microtome knives. This stops rapid deterioration of the disposable

blade.

2. SYMBOLS

Dangers, warnings and cautions are marked by this symbol

Special instructions regarding the operation of the

instrument are marked by this symbol

Mechanical components that can lead to injuries during

operation are marked with this symbol.

3. SAFETY NOTES

The Tanner Scientific open top cryostat TN50 is provided with the following safety

features:

TN50

Hand wheel stop



Finger protection for Blade-holder



The institution which owns the unit and the persons working with the unit, servicing or

repairing it are responsibe for a hazard-free use.

Содержание TN50

Страница 1: ...OPERATING INSTRUCTIONS Cryostat TN50 INS2000GB 2018 10 003 00 ...

Страница 2: ...Operating Instructions TN50 2 ...

Страница 3: ...S 4 3 SAFETY NOTES 4 4 COMPONENTS 8 5 SPECIFICATIONS 9 6 UNPACKING AND INSTALLATION 11 7 INITIAL OPERATION 14 8 OPERATION OF MICROTOME 20 9 OPERATION OF CRYOSTAT 28 10 CLEANING AND MAINTENANCE 33 11 SERVICE 37 12 OPTIONAL ACCESSORIES 38 13 WARRANTY 39 14 DISPOSAL 39 ...

Страница 4: ...he specimen which avoids rubbing on the disposable blades or microtome knives This stops rapid deterioration of the disposable blade 2 SYMBOLS Dangers warnings and cautions are marked by this symbol i Special instructions regarding the operation of the instrument are marked by this symbol Mechanical components that can lead to injuries during operation are marked with this symbol 3 SAFETY NOTES Th...

Страница 5: ...ition before working with the blade or specimen before changing the specimen during break time Hand wheel stop released Movement Hand wheel stop activated No movement The hand wheel can be stopped in any position For this purpose move the stop lever towards the centre To release the stop please turn the lever towards the outside again ...

Страница 6: ...N50 6 3 2 FINGER PROTECTION Always use the finger protection red lever Before you start any work with the blade or specimen Before changing the specimen During work break Finger protection released Finger protection activated ...

Страница 7: ...ated fuse for the unit Do not connect another unit to the same power circuit Before turning on the instrument check if the voltage of the mains supply is identical with the name plate of the unit 3 4 WORKING WITH DISPOSABLE BLADES Disposable blades have extremely sharp edges and this can lead to injuries Please be extremely careful when handling disposable blades Do not place disposable blades at ...

Страница 8: ...with the following standard components TN50 Basic unit with refrigeration system Semi automatic rotary microtome Operation manual clear cut Brush Anti roll plate Ready to use 1x Bottle cryostat low temperature oil 4x 125 ml CryoGlue embedding medium 1 Box of disposable blades 50 pcs Set of Allen keys ...

Страница 9: ...ondensing Humidity during storage 60 Cryochamber Temperature range 0 C to 35 C selectable in 1 K steps Environment 20 C Cooling power 514 W Switch off pressure 25 bar Current 6 45 A Cooling gas R 449 A 320 g Chamber defrost Automatic defrost hot gas defrost time selectable 1 3 times per day or manual defrost on request Automatic stop at 5 C chamber temperature Freezing bar Minimal temperature 10 K...

Страница 10: ... 50 µm in 2 µm steps 50 100 µm in 5 µm steps Horizontal movement 28 mm Vertical movement 58 mm Retraction during upward stroke 0 200 µm free selectable defined steps Trimming 0 750 µm free selectable defined steps Specimen orientation 8 x and y axes 360 z axes Cryostat Dimensions L x W x H 680 mm x 760 mm x 1110 mm 27 x 30 x 44 Weight without accessories 127 kg 280 lbs ...

Страница 11: ...transport Note this on the shipping documents and check the shipment for possible damage Open the cardboard box from the top and remove the accessories together with the supporting foams Then remove the cardboard box Remove the screws on the two fixation brackets that hold the instrument on the wooden pallet Swing out the ramp on which the instrument can be rolled down off the transport pallet To ...

Страница 12: ...t be reached easily Relative humidity lower than 60 A minimum distance between wall and rear of the instrument of 10 20 cm 8 10 inches should be guaranteed i High room temperatures and high humidity influence the cooling capacity of the instrument and will lead to ice forming inside the instrument 6 2 INSTALLATION For opening the chamber push the window to the top Remove the transport security und...

Страница 13: ...hand wheel lever that is folded away for transport reasons Before you start any work unfold the hand wheel lever by simply pulling the lever into a horizontal position For folding the lever back pull the horizontally orientated lever and fold it away Hand wheel lever in folded position Hand wheel lever unfolded ...

Страница 14: ...the trimming thickness the current chamber temperature the target chamber temperature the status of the quick freeze unit optional CUT 7 µm Trim 19µm Cha 20 C 22 QF 0 7 2 CONTROL PANEL Software settings and motor operation can be operated with the control panel on the left to side of the instrument TN50 Control Panel ...

Страница 15: ... Start defrost Contrast TIME TUE 13 05 05 To move further up in the main menu list Press Light Auto Light duration 10 m Chamber incr 15 C Retraction 5 µm To change values select a setting Press to open the setting Change entries by pressing or Store new settings by pressing Light duration 10 m Chamber incr 15 C Retraction 5 µm Start defrost Press to return to the main menu CUT 7 µm Trim 19µm Cha 2...

Страница 16: ...ministrators Enter PIN 0000 Select defrosting programs 1 to 3 and set a time for the automatic defrosting programme e g each day at 12 00 h and at 23 00 h in this example Defr Start 1 12 00 Defr Start 2 23 00 Defr Start 3 __ __ Disinf Req __ __ Select temperature increase start and set a starting time for the automatic power saving mode e g start at 21 00 h in this example Disinf delay 10 h Incr s...

Страница 17: ... the days at that the automatic power saving mode will be active for 24 hours e g Fridays and Saturdays in this example Disinf delay 1 2 _ _ _ _ _ Incr start 21 00 Incr end 04 00 Incr days _ _ _ _ 5 6 _ 1 Monday 2 Tuesday 3 Wednesday 4 Thursday 5 Friday 6 Saturday 7 Sunday ...

Страница 18: ...the optional fast cooling peltier system its function can be activated or deactivated The default setting for automatic deactivation of the quick freeze is set to 30 minutes The timer for automatic deactivation can be set by an authorized service technician UV disinfection ON OFF If the instrument is equipped with the optional UV C disinfection system its function can be activated or deactivated T...

Страница 19: ...is activated Trim latching ON OFF If Trim latching mode is activated trimming function is activated and deactivated by pressing once If Trim latching mode is deactivated trimming function is activated by continuously pressing Contrast Display contrast Time Day and Time Language A language can be selected out of a list of available languages Display version Display of software version Program setti...

Страница 20: ... 1 Activate hand wheel lock 2 Remove finger protection 3 Loosen the blade fixation by turning the right lever counter clockwise 4 Remove Insert blade from one side Tighten the blade fixation by turning the right lever clockwise Loosening of blade fixation and insertion of new blade Fixation of blade 2 4 3 ...

Страница 21: ... the blade holder A tilt towards the object holder increases the cutting angle clockwise turn of the knob a tilt away from the object holder decreases the cutting angle counter clockwise turn of the knob Tighten the blade holder fixation by turning the right lever on the blade holder base clockwise Release hand wheel lock for cutting Loosening of blade holder fixation and adjusting new cutting ang...

Страница 22: ...t is frozen mat surface or pressure 8 3 INSERTION OF OBJECT HOLDER 1 Turn hand wheel and move object holder to its highest position and activate the hand wheel stop 2 Release the object holder fixation by turning the fixation lever counter clockwise 3 Remove Insert object holder 4 Tighten the object holder by turning the fixation lever clockwise Please pay attention that under all circumstances du...

Страница 23: ...on lever on the left side of the sample holder counterclockwise 2 To adjust the orientation swing the right orientation screw Fix the sample orientation by turning the fixation lever on the left side of the sample holder clockwise Release the handwheel lock to cut the samples Release the sample orientation and orientation of the sample Fix the sample orientation i Levers must be up 4 1 2 ...

Страница 24: ... ADJUSTMENT FOR CUT To change the cutting thickness press or to increase or decrease the value 8 9 TRIMMING OF SPECIMEN Insert object holder Orientate specimen as desired Deactivate the hand wheel stop Release finger protection If Trim latching mode is activated trimming function is activated and deactivated by pressing once Activation of trimming function is displayed as illustrated TRIM Trim 19µ...

Страница 25: ...g or The selected section thickness is indicated in the display e g 7 µm in this example CUT 7 µm Trim 19µm Cha 20 C 22 QF 0 Turn hand wheel until some even sections have been obtained Clean the disposable blade always away from the cutting edge with a cold brush Position anti roll plate onto the disposable blade and cut If necessary readjust the height of the guide plate Leave hand wheel with han...

Страница 26: ...right away from blade For pick up of the samples turn the anti roll plate sidewards with the help of the knob In this case make sure that the anti roll plate is folded into the horizontal position when it is unfolded in order to prevent a one sided warm up Horizontal adjustment of anti roll plate i For height adjustment of the anti roll plate it is recommended to adjust the correct height during c...

Страница 27: ... 25 Brain 7 to 10 Breast with fat 25 to 30 i For tissue which has to be cut considerably below normal chamber temperature e g fatty tissues and in order to avoid cooling artefacts an additional bench top CO2 freezer is available Exceptionally freezing spray can be used Breast without fat 16 to 20 Cartilage 13 to 20 Fat 30 to 40 Heart 20 to 25 Intestinal 13 to 20 Kidney 13 to 20 Larynx 13 to 16 Lip...

Страница 28: ...ivated The default setting for the UVC light duration and the automatic deactivation is set to 30 minutes The timer can be set by an authorized service technician i For safety reasons UVC light will be deactivated upon opening of the lid of the cryochamber The UVC disinfection will be aborted and has to be activated again for surface disinfection The default setting for automatic deactivation is s...

Страница 29: ... be reached this place will be needed to connect the UV lamp with the connecting plug Pic 2 3 Picture 4 The connector can be plugged into two directions of polarity a switch is excluded Picture 5 Picture 6 Picture 7 Once the plug is connected to the UV lamp the final step can be done now and the UV lamp unit can clip into the retaining clips ...

Страница 30: ...automatic deactivation of the quick freeze is set to 30 minutes The timer for automatic deactivation can be set by an authorized service technician 9 3 HEAT EXTRACTOR For a more rapid freezing process with improved structural preservation of tissues and a flattened block surface the instrument is equipped with a heat extractor Apply the heat extractor on top of the specimen during the freezing pro...

Страница 31: ... through the evaporator All ice which has been built up is melted the water drops into the condensate bottle The defrosting process ends at a temperature of 20 C on the evaporator The chamber temperature remains in the minus range so that samples which are stored there do not thaw i The defrost process starts automatically at midnight every day To change this time please refer to section 7 3 i Bef...

Страница 32: ...order to avoid a decision Switch on the instrument i Before starting a complete defrost of the instrument assure that all samples have been removed from the cryochamber Before switching on the instrument ensure that the instrument is completely dry otherwise ice built up can damage mechanical parts 9 6 EMPTYING THE CONDENSATE BOTTLE The unit is equipped with a condensat bottle for defrosting water...

Страница 33: ... without removing the microtome from the cryochamber When disinfecting the instrument take appropriate protective measures gloves mask protective clothing etc Wear protective clothing and disposable gloves according to Good Laboratory Practices When using detergents and disinfectants please comply with the safety precautions of the disinfectant manufacturer The integrated anti roll plate of the bl...

Страница 34: ...of blade holder see section 10 3 Cleaning of window Monthly Check of temperature sensor in cryochamber Cleaning of cryostat ventilation see section 10 4 Yearly Complete Service performed by authorized Tanner Scientific service technician check of all functions complete defrost removing of microtome oiling of movable parts check of cooling system check of driving system complete disinfection cleani...

Страница 35: ...ade holder using the ejection mechanism Loosen the upper part of the blade holder by turning the upper lever counterclockwise Slide the top of the blade holder to the right Remove the pressure plate and clean the blade holder with a brush or cloth Reassemble the pressure plate and the lever in reverse order and release the handwheel lock for cutting Pull out the lever and release the upper blade h...

Страница 36: ...an negatively affect the cooling performance of the instrument A cleaning of the cryostat ventilation is recommended monthly Remove the front panel of the unit by unscrewing the 8 screws four on each side Remove the dust with a vacuum cleaner A cleaning of the cryostat ventilation is recommend ded monthly We suggest using a vacuum cleaner and a brush for cleaning ...

Страница 37: ...on available Complete contact details Type of instrument and serial number Place of instrument and name of user Purpose of service call Delivery date of the unit If it is necessary to return the instrument it must be cleaned and disinfected before delivery It must be returned in its original packing If the instrument or parts thereof are sent back in a dirty or non disinfected condition Tanner Sci...

Страница 38: ...IONAL ACCESSORIES Disposable Blade Holder low profile Disposable Blade Holder high profile 2x Quick freezing positions UV disinfection Heat extractor block Cork plate 100 pcs Ø 20 mm clear cut Brush Anti roll plate disposable blade holder ...

Страница 39: ...rument is manufactured under an ISO 9001 quality management system Unauthorized modification or repair by third party persons will void the warranty Only original Tanner Scientific spare parts must be used Guarantee claims can be put forward only if the instrument is used according to this manual and for the purpose described Mistakes and errors which occur because of improper use cannot be accept...

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Страница 42: ...Tanner Scientific 12210 Rangeland Parkway 34211 Bradenton FL US Tel 888 708 5233 sales tannerscientific com http www tannerscientific com ...

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