Instrument Operating Manual
CyFlow® Cube 8 Rev-27_2015-07-02
Sysmex Partec GmbH
Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · [email protected] ·
www.sysmex-partec.com
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9.4.3 CyFlow
®
Cube 8 Optics
Laser / Output
Red Diode Laser:
25 mW at 635 nm/40 mW at 640 nm
Green NdYAG:
30 mW to 100 mW at 532 nm
Blue laser diode
50 mW at 488 nm/200 mW at 488 nm
Violet Diode Laser:
100 mW at 405 nm
Near Ultra-Violet
Diode Laser:
16 mW at 375 nm
Yellow Diode Laser:
100 mW at 561 nm
Orange Diode Laser:
50 mW at 594 nm
Detectors
1 to 8 (FSC, SSC, FL1, FL2, FL3, FL4, FL5, FL6)
Filters
Standard setup and filters for all parameters according to laser
configuration
Optical Coupling
Standard objective mount with high numerical aperture objective,
high numerical aperture immersion gel coupling, e.g. for detection
of weak cytokines (option)
Excitation Optics
Elliptical 15 µm x 100 µm at 488 nm, other beam geometries upon
request
9.4.4 CyFlow
®
Cube 8 Fluidics
Fluidic System
Completely closed system for sheath water and sample volumes;
no fluid droplets or aerosols generated nor released from the
instrument.
Flow Cuvette
Synthetic quartz flow cuvette (350x 200 µm) for laminar sample
transport with sheath fluid fluorescence, forward and side scatter
light detection
Sample Delivery
Computer controlled precision syringe pump for contamination-free
sample transport.
Built-in vacuum pump for waste container. Vacuum pressure is
adjustable (Computer controlled).
Sampling Volume
Continuous up to 1200 ml.
200 µl for electrode based precision absolute counting, Other
counting volumes upon request
5
–1000 µl for syringe based precision absolute counting
Flow Rates
1) Sample volume speed adjustable continuously between 0.1 and
20 µl/s
2) Sheath fluid flow continuously adjustable in expert mode
Fluidics Volume
2x 1-litre integrated reservoirs for sheath fluid and waste
BioSafety System
Avoids sample droplets and sample cross contamination (computer
controlled).
