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3. Image Acquisition
NuGenius/ N User manual
17
Chapter Three - Image Acquisition
3.1 Capturing an image of an agarose EtBr or similar gel
Step 1 – Position sample and choose lighting
Press the 'Live' button and then position the sample on the centre of the
Transilluminator and close the door. Select ‘UV TRANSILLUMINATOR’ from the
lighting options.
Step 2 – Adjust lens
Adjust the aperture, zoom and focus settings until a suitable image is displayed,
preferably with the sample filling the screen for maximum resolution.
Please note that opening the aperture too far may result in areas of the sample
being saturated.
You can select to display any saturation on screen by pressing the ‘DISPLAY
SATURATION’ button. Saturated areas will appear orange.
Step 3 – Set exposure time
The exposure time can either be set manually or automatically. Adjusting the
exposure time will alter the brightness of the image. To manually increase or
decrease the exposure time, use the following icons.
Click on the or to increase or decrease the exposure time, or
alternatively, click on the time to set it manually.
Set an exposure time you are happy with and press ‘CAPTURE’.
Alternatively, the auto-exposure function sets the exposure time to a level that
ensures no saturation of the image occurs. To use the auto-exposure function press
‘AUTO’.
You can then save or print the image.