14
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Insert the phase contrast slide into condenser slot until it clicks into
position. For example, a 10x Ph1 objective with Ph1 phase slide.
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Remove the eyepieces and replace them with the centring telescope.
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Bring the centering telescope into focus, so that the black ring of
objective phase plate and segmented phase annulus of phase slide are
in focus.
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Use the knurled screws to center the black ring of phase plate and
phase annulus of phase slide.
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Phase contrast is ready when the light of segmented phase annulus sits
on the black ring of phase plate.
Objective phase plate ring
Phase slide annulus