12
Alignment of the Phase Annulus:
To align each annulus, begin by
removing the eyepiece from the eyepiece tube that does
not
have the
diopter adjustment (the right eyepiece). (Please note: Should the
eyepiece be l
ocked onto the microscope, use a jeweler’s screwdriver to
loosen the eyepiece set screw to enable you to remove the eyepiece).
Place the centering telescope (CT, included with the phase system) into
the eyepiece tube:
The condenser and stage should remain in the highest position and
the iris should be fully opened. Do not adjust the focus controls
Loosen the thumb screw on the centering telescope (CT). While holding
the knurled locking screw with one hand, grasp the very top portion of
the centering telescope with the other hand, look through the eyepiece of
the centering telescope while slowly sliding the telescope tube out until
the phase ring in the
objective
is in focus. Tighten the knurled locking
screw
.
10X Phase:
Begin by placing the condenser disk of the phase condenser
on the
10
position. Ensure that i
t is “clicked” into position the 10X phase
objective is in the optical path.
Using only the condenser focusing control knob, focus the bright annuli
ring located in the
phase condenser
. Now observe the two rings in the
field of view. The dark larger annulus ring is located it the objective lens.
The bright smaller annulus ring is located in the phase condenser. When
properly aligned, these will appear as superimposed rings. Incorrect
alignment will require that the annuli be re-centered.
