10
Phase Contrast
is defined as “
an optical technique used to better
view the structure of transparent objects whose differences in
thickness result in a difference in the phase of the transmitted
light.”
This is also referred to as “optical staining”. The
condenser should be at the highest position and the iris fully
open. In addition, it will be necessary for the phase contrast to
add a clear green filter. The filter optically stains the specimen
allowing structures that are now seen in phase contrast to be
further enhanced. Place the green filter in the swing-out filter
carrier.
Please note
: For models that have LED illumination, an
additional swing-out filter carrier is provided. LED illumination
provides a very white light that needs to be softened with a
neutral diffusing filter when using the 10X objective. Place the
neutral diffusing filter in the secondary filter carrier.
Rotate the disc to the desired phase magnification (10, 20/40, or
100) to match the objective you are using. (Please note: the 4x
objective included with the system is
not
phase)
10x and 40x Objectives:
Adjust and focus as normal. Iris must
be fully opened
100x Objective
: Make sure the iris is fully open and the
condenser is at the highest position. The green filter may or may
not be used, depending on the desired result upon evaluation of
highly magnified specimens. It is highly recommended that
proper immersion oil be used on the surface of the slide. Use of a
dry P100X phase objective may not produce the desired results.
Please be sure to clean the oil from the lens surfaces when you
are finished. Please consult your Swift Use and Care manual for
proper objective cleaning and care instructions.
