Sutter Instrument P-97, Инструкции по эксплуатации

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3.6.3 Box Filament
The box-shaped heating filament is recommended as the standard for most applications. The
box filament heats the glass in a more symmetrical fashion than other filaments (such as the
trough filament), so that the pipettes produced tend to be shorter and more straight and
concentric. It delivers more heat to the glass resulting in faster heating without the necessity
of increasing the temperature of the filament.
Figure 3-13. End view of box filament and glass.
Note: The Ramp Test value with a box filament will be higher than that with the trough
filament, thus program HEAT values will be correspondingly higher in order to reach similar
operating temperatures.
Box filaments are recommended for the following micropipettes:
Patch pipettes using thick-walled glass
Microelectrodes used for slice preparations where long, parallel walls would aid
penetration
Microinjection needles for transgenic research
Microdissection tools
Thick or multi-barreled glass
Aluminosilicate glass
The box filament has two primary limitations.
First, it requires more current to heat to a given temperature than the same size
trough filament. Thus, it is possible to use wider trough filaments without exceeding
the maximum current capacity of the puller.
Second, the box configuration reduces the cooling effect of the air jet. For this reason,
velocity settings often lowered.
3.6.3.1 Positioning
When using a box filament, the glass tubing should be centered vertically and horizontally
(Figure 3-12). See section Error! Reference source not found. for adjustments and alignment.
P-97 FLAMING/BROWN MICROPIPETTE PULLER OPERATION MANUAL – REV. 2.43 - DOM (20161118)