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Stat Fax 303 Plus Operator’s Manual Rev. J
Awareness Technology, Inc.
2.2.8 Uptake Mode
The Uptake mode (UPT) is a modified Calibrator mode often required for uptake assays. Be
careful to use your cutoff options correctly in this mode. In this mode, a factor is calculated by
multiplying the absorbance of a single calibrator by its concentration. This factor is then divided
by each subsequent absorbance to calculate the concentrations of unknown samples. No blank
may be used and the calibrator must be in the first well. Note that this mode will neither print nor
store the curve.
After filter, duplicates, and unit selections, the display prompts
“CAL1=”
. Type in the calibrator
concentration (up to 999999, up to two places after the decimal point for CAL1 < 1000) and
press ENTER.
The display will then prompt
“Cal Limits Y/N”
. Select NO to continue, or select YES to enter
absorbance limits for the calibrator, the display will prompt
“Cal 1 >=”
. Enter the minimum
acceptable absorbance value for the calibrator.
“Set Carrier to X”
,
“Then Press Enter”
is displayed, where X is either 1 or A, depending on the
strip type. Load the first strip into the right-most carrier position and press ENTER. While the
strip is being automatically positioned for reading, the display will indicate
“Positioning Strp”
.
The instrument will be ready for the next strip when it displays
“Set Carrier”
,
“Then Press
Enter”
. To continue reading in the Uptake mode, load the next strip and press the ENTER key.
Press CLEAR twice to cancel the mode.
2.3 User Test Menu
This instrument offers the operator 8K bytes of non-volatile memory. This is user programmable
memory (enough for about 31 tests) that will not be lost when the instrument is turned off. Test
selections may be stored so that setup time is significantly reduced when the same assay is
repeated. Curves are stored in all modes except Absorbance and Uptake. The time and date are
maintained using this same type of memory.
Items in non-volatile memory may be entered, recalled, and deleted from memory by the
operator following the instructions in this section.
There are several test numbers reserved for factory use. The user should not come across these
in normal use; however, if one is inadvertently selected, it may be aborted by turning the
instrument off and then back on.
2.3.1 Storing a Test
Tests are stored in a user test menu by number, with an option to store by name. The first test
that you store will be test number 1; the next test will be test number 2, and so on. When you
want to recall a test, you will be asked to enter the test number. A running list or log book should
be kept with your instrument to help you identify your numeric entries. A sample format for this
log can be found at the end of this manual. Each time you store a test, the instrument will assign
the next available test number. Remember to update your log. The instrument will record the
date and time that each test was stored or last modified.
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