19
The equation is very simple:
Dilution Ratio Factor = # of mLs of diluent
# of mLs of sample added
Then add 1 to get the Dilution Factor
DOUBLE DILUTION
For high-concentration samples, this procedure will ensure that a large enough,
representative sample is taken to provide a realistic size and count analysis.
STEP 1
Start with 99 mL of particle free diluent in the beaker. (Note: it greatly
simplifies calculations by using 99 instead of 100 mL).
STEP 2
Add 1 mL of sample and thoroughly mix. Call this Sample “A”.
(Note: Dilution factor is 100:1).
STEP 3
Place another beaker of 99 mL of diluent on the particle counter and
check that it is clean, with less than 30 counts >1 µm
STEP 4
Add 1 mL of Sample “A” and stir.
STEP 5
Continue to add precise amounts until total counts >1 µm are 5-600/mL
STEP 6
Calculate dilution ratio as follows (for Sample “A”):
First dilution
Dilution ratio is 99+1
(Dilution factor is 100:1 for Sample “A”)
1
Final dilution
100 x 100
Number of mLs added in Step 5
Example:
If 1 mL of “A” is added in second dilution state, the dilution factor is
100 x 100 = 10,000
1
If 2 mL of “A” are added, the dilution factor is
100 x 100 = 5,000
2
Содержание PC-2300
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